Characterization of Protein Kinase C in Cisplatin Sensitive and Resistant Human Cervical Cancer HeLa Cells
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Abstract
Mohanty, S., Characterization of protein kinase C in cisplatin sensitive and resistant human cervical cancer HeLa cells. Master of Science (Microbiology and Immunology), December, 2000. 37 pp., 11 illustrations, bibliography, 27 titles. Signal transduction plays a crucial role in carcinogenesis. A defect in signaling, by evading cell death or promoting cell proliferation, may result in neoplastic transformation or protection of cells from the cytotoxicity of anticancer drugs. Therefore, in order to understand the complex mechanism of drug resistance, it is relevant to probe into the important signal transduction pathways. Protein kinase C, a key signal transducer, influences cisplatin sensitivity in many cell lines. We examined whether or not the PKC signal transduction pathway is affected during development of resistance to cisplatin by tumor cells. PKC activators increased cisplatin sensitivity in both parental and cisplatin-resistant cells. Western blot analysis showed a slight decrease in cPKCα and nPKCε, an evaluation in nPKCδ and no change in the abundance of PKCϚ in HeLa/CP cells compared to HeLa cells. Though TPA-induced translocation of PKC isoforms was identical in both cell lines, down regulation of PKCδ was defective in resistant cells. Therefore, a deregulation in PKCδ was associated with cisplatin resistance.
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Cell and Developmental Biology
Cell Biology
Cells
Cellular and Molecular Physiology
Life Sciences
Medical Cell Biology
Medicine and Health Sciences
Oncology
Other Cell and Developmental Biology
Other Pharmacy and Pharmaceutical Sciences
Pharmacology
Pharmacy and Pharmaceutical Sciences
Toxicology
Characterization
protein kinase C
cisplatin sensitive
cisplatin resistant
human cervical cancer
HeLa cells
signal transduction
carcinogenesis
cell death
cell proliferation
anticancer drugs
cytotoxicity
PKC