Evaluation of the PowerSeq™ Auto System by Massively Parallel Sequencing

Massively parallel sequencing (MPS) is potential technology for STR typing by forensic laboratories and that some of the CE-based limitations may be overcome by MPS. In this study, the PowerSeq Auto System (Promega) containing 23 STR loci and Amelogenin, was evaluated by MPS. The PCR products were size selected using the MinElute PCR Purification Kit (Qiagen). DNA libraries were normalized, pooled and sequenced on the MiSeq (Illumina; 2 x 250 bp). This multiplex STR system was tested for sensitivity of detection based on input DNA. The result showed that a broad range of the quantity of PCR products could be used for library preparation. In mixture study, the partial profile of minor contributor could be detected up to 19:1 mixture. These studies indicate that PowerPlex Fusion STR system and the Illumina MiSeq system can generate reliable DNA profiles with the types of samples and amounts of input DNA that are relevant to forensic analyses.