: Comparative Microbiome Analysis of Breast Cancer Tissue in Black Non-Hispanic and White Non-Hispanic women.

Abstract

Title: Comparative Microbiome Analysis of Breast Cancer Tissue in Black Non-Hispanic and White Non-Hispanic women. Background: Triple negative breast cancer (TNBC), abreast cancer disparity (BCD) with an especially aggressive subtype, is more prevalent among Black Non-Hispanic (BNH) women as compared to White Non-Hispanic (WNH) women.BNH women exhibit BCD as shown by higher level of mortality rate than WNH women. Studies have shown that the breast microbiota may have a major influence on breast carcinogenesis. Methods:We have characterized the microbiome of breast cancer and normal tissue samples from the same patients using 16S rRNA gene targeted sequencing. Two distinct breast tumor types were included in the study: TNBC and triple positive breast cancer (TPBC). The data were analyzed for microbiota composition, abundance, and diversity. Results:Our preliminary analysis revealed that both richness and evenness of the microbial community (as measured by alpha diversity metrics), of normal breast tissue was significantly different in comparison to the matched tumor breast tissue. The microbiota richness in BNH TNBC tumor was lower when compared to that of the matched normal breast tissue. In contrast, the microbial richness in WNH TNBC tumor was higher when compared to that of the matched normal breast tissue. The multivariate analysis of beta diversity revealed adistinct clustering of the microbial communities between BNH TNBC tumor and the matched normal tissue. Conclusions:Our findings demonstrated that BNH and WNH racial groups exhibit distinct alpha and beta microbial diversity patterns in normal and cancer breast tissue. Sponsor:Research reported in this publication was supported in part by the National Cancer Institute of the National Institutes of Health under Award Number R01CA220273 02 (to Dr. J.K. Vishwanatha)and by the National Institute On Minority Health and Health Disparities of the National Institutes of Health under Award Number S21MD012472 ( to J.K.Vishwanatha). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. IRB Project #: 2009-001 IBC/p/JV-2017-1