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dc.contributor.advisorJoseph Warren
dc.creatorAriyo, Bolanle
dc.date.accessioned2019-08-22T20:25:21Z
dc.date.available2019-08-22T20:25:21Z
dc.date.issued2004-08-01T00:00:00-07:00
dc.date.submitted2014-05-01T06:48:05-07:00
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28347
dc.description.abstractAriyo, Bolanle. Amplification of Mitochondrial DNA Regions HVI and HVII in its Entirety and Reducing Cycle Sequencing Reactions. Master of Science (Forensic Genetics), August 2004, 46 pages, 10 figures, 7 tables, 18 references. Mitochondrial DNA is widely used in the forensic community because of its high copy number in cells, location, and mode of inheritance. Yet this method of analysis is expensive, time consuming, and labor intensive, therefore labs should take steps to improve the procedure of mtDNA analysis. This study is performed to validate the use of amplifying HVI and HVII region in its entirety (2 primer sets) for use in reference samples. Amplification performed using primers F15989-R16410 (HVI) and F73-R340 (HVII). The current method of amplification is 4 primer sets at full cycle sequencing reactions. The cost of Cycle Sequencing Kit is also expensive, therefore performing half and quarter reactions would be beneficial in reducing the amount of kit consumed. To validate the use of reducing cycle sequencing reactions, half and quarter cycle reactions were performed using 2 and 4 primer sets. Results demonstrate that sequence data for reducing cycle sequence data is consistent with the sequence data using the current method. Results also show that sequence data obtained using two primer sets was consistent with sequence data amplified by the current method with the exception of two samples at length heteroplasmy polyctosine regions.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectAmplification
dc.subjectmitochondrial DNA
dc.subjectHVI
dc.subjectHVII
dc.subjectCycle Sequencing Reactions
dc.subjectforensics
dc.subjectmtDNA analysis
dc.subjectamplification
dc.subjectcycle sequence data
dc.subjectlength heteroplasmy polyctosine region
dc.titleAmplification of Mitochondrial DNA Regions HVI and HVII in its Entirety and Reducing Cycle Sequencing
dc.typeProfessional Report
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science
dc.contributor.committeeMemberJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.type.materialtext
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