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    Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Fornesic and Paternity Laboratories

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    Date
    2002-08-01
    Author
    Johnson, Cassie L.
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    Abstract
    Johnson, C., Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Forensic and Paternity Laboratories. Master of Science (Forensic Genetics), August, 2002. 94 pp., 6 tables, 7 figures, references, 21 titles. Y-chromosome microsatellite markers (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of males. The present study validated a Y-STR 10-plex on the ABI Prism 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid GeneScreen. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. The mutation rate varied between 0-0.238 (+/- 0.045, 95% confidence interval). The present study demonstrated that the 10-plex is sensitive to 0.75 ng and that female samples mixed with male samples did not interfere with Y-STR haplotyping. A population database of 525 males was developed and subsequently analyzed. Three instances of locus multiplication were observed, two at DYS19 and one at DYS435. Overall haplotype diversity was 0.996, suggesting that the 10-plex can efficiently distinguish male Y-STR profiles.
    Subject
    Cell and Developmental Biology
    Computational Biology
    Equipment and Supplies
    Forensic Science and Technology
    Genetics
    Genetics and Genomics
    Genetic Structures
    Health Information Technology
    Investigative Techniques
    Life Sciences
    Medicine and Health Sciences
    Other Genetics and Genomics
    Validation
    y-chromosomal short tandem repeat
    10-plex
    forensic laboratories
    paternity laboratories
    rape
    Y-chromosome microsatellite markers
    Y-STRs
    genetic identification
    males
    Orchid GeneScreen
    ABI Prism 3100 Genetic Analyzer
    mutation rate
    haplotype diversity
    population database
    URI
    https://hdl.handle.net/20.500.12503/29124
    Collections
    • School of Biomedical Sciences
    • Theses and Dissertations

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