Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Fornesic and Paternity Laboratories
Abstract
Johnson, C., Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Forensic and Paternity Laboratories. Master of Science (Forensic Genetics), August, 2002. 94 pp., 6 tables, 7 figures, references, 21 titles. Y-chromosome microsatellite markers (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of males. The present study validated a Y-STR 10-plex on the ABI Prism 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid GeneScreen. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. The mutation rate varied between 0-0.238 (+/- 0.045, 95% confidence interval). The present study demonstrated that the 10-plex is sensitive to 0.75 ng and that female samples mixed with male samples did not interfere with Y-STR haplotyping. A population database of 525 males was developed and subsequently analyzed. Three instances of locus multiplication were observed, two at DYS19 and one at DYS435. Overall haplotype diversity was 0.996, suggesting that the 10-plex can efficiently distinguish male Y-STR profiles.
Subject
Cell and Developmental Biology
Computational Biology
Equipment and Supplies
Forensic Science and Technology
Genetics
Genetics and Genomics
Genetic Structures
Health Information Technology
Investigative Techniques
Life Sciences
Medicine and Health Sciences
Other Genetics and Genomics
Validation
y-chromosomal short tandem repeat
10-plex
forensic laboratories
paternity laboratories
rape
Y-chromosome microsatellite markers
Y-STRs
genetic identification
males
Orchid GeneScreen
ABI Prism 3100 Genetic Analyzer
mutation rate
haplotype diversity
population database
Computational Biology
Equipment and Supplies
Forensic Science and Technology
Genetics
Genetics and Genomics
Genetic Structures
Health Information Technology
Investigative Techniques
Life Sciences
Medicine and Health Sciences
Other Genetics and Genomics
Validation
y-chromosomal short tandem repeat
10-plex
forensic laboratories
paternity laboratories
rape
Y-chromosome microsatellite markers
Y-STRs
genetic identification
males
Orchid GeneScreen
ABI Prism 3100 Genetic Analyzer
mutation rate
haplotype diversity
population database
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