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    Oxidative Stress Alters IP3 Receptor Function in the Neuronal Cell Line HT22

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    Longoria_OxidativeStressAltersIP3.pdf (17.94Mb)
    Date
    2008-05-01
    Author
    Longoria, Sandra
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    Abstract
    Sandra Longoria., Oxidative Stress Alters IP3 Receptor Function in the Neuronal Cell Line HT22, Master of Science (Biomedical Sciences), May 2008, 72 pp., 25 Figures. Oxidative stress contributes to the genesis of several neurodegenerative disorders such as Alzheimer’s Disease (AD). Oxidants such as, tert-butyl hydrogen peroxide (tBHP), have been used in in vitro models of neurodegeneration to induce oxidative stress. Small changes in the regulation of the intracellular calcium (Ca2+) concentration can contribute to brain aging and increase vulnerability of neurons to cellular and functional damage in neurodegenerative diseases. In neurons, inositol 1, 4, 5-trisphosphate (IP3) is a second messenger that is generated through receptor activity at the plasma membrane. IP3 receptors (IP3R) are located on endoplasmic reticulum (ER) membranes and are intracellular calcium channels (ICC) that release Ca2+ into the cytoplasm in response to activation by their ligand IP3. The goal of the present study was to measure the contribution of ICCs to Ca2+ dysregulation in neurons experiencing oxidative stress. I tested the hypothesis that oxidative stress induced with tBHP causes increased intracellular Ca2+ release via activation of IP3 receptors. I used the murine hippocampal cell line HT22, as a model for neuronal oxidative stress. Immunocytochemistry and Ca2+ imaging experiments were performed to identify areas of altered IP3R expression and activity under normal conditions and induced oxidative stress. tBHP treatment increased expression and Ca2+ release activity of neuronal IP3 receptors. My findings support that oxidative stress as seen in a number of neurodegenerative diseases negatively affects regulation of Ca2+ release through increased expression and activity of IP3 receptors.
    Subject
    Cell and Developmental Biology
    Cell Biology
    Cells
    Cellular and Molecular Physiology
    Life Sciences
    Medical Cell Biology
    Medicine and Health Sciences
    Molecular and Cellular Neuroscience
    Neuroscience and Neurobiology
    Neurosciences
    Other Cell and Developmental Biology
    Oxidative stress
    IP3 Receptor Function
    neuronal cell line HT22
    Alzheimer’s Disease
    neurodegenerative diseases
    intracellular calcium channels
    Ca2+
    neurons
    tBHP
    tert-butyl hydrogen peroxide
    URI
    https://hdl.handle.net/20.500.12503/29152
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