Horse Serum High Density Lipoproteins as Drug Transporters
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Johnson, Shemedia J., Horse Serum High Density Lipoproteins (HDL) as Drug Transporters. High-density lipoproteins (HDL) are complex particles composed of specific proteins and lipids that facilitate blood and tissue cholesterol homeostasis by transporting excess peripheral cholesterol to the liver. In association with cholesterol ester transfer protein (CETP) and the enzyme, lecithin: cholesterol acyltransferase (LCAT), HDL contributes to the transport of hydrophobic lipids, including cholesterol ester and triglycerides through the blood. The studies presented here involve the evaluation of horse serum HDL as a carrier of water insoluble drugs and an improved process to isolate and purify horse serum HDL utilizing hydrophobic affinity chromatography. Dilauryl fluorescein (DLF) has been chosen as a model compound for the study of horse HDL as a drug carrier. The prepared HDL/DLF particles have similar flotation densities and size properties to native horse serum HDL. The amount of DLF incorporated into HDL is 30μg/mg protein. Various cancer cell lines internalized DLF from horse HDL/DLF particles successfully. While human plasma contains cholesterol ester transfer protein (CETP), horse plasma does not. Horse plasma/serum can be supplemented by human plasma to study the role of CETP in drug transport and the stability of the horse HDL/drug complex.
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Cell and Developmental Biology
Chemicals and Drugs
Circulatory and Respiratory Physiology
Hemic and Immune Systems
Life Sciences
Medicinal Chemistry and Pharmaceutics
Medicine and Health Sciences
Other Pharmacy and Pharmaceutical Sciences
Pharmacology
Pharmacy and Pharmaceutical Sciences
Horse serum high density lipoproteins
HDL
drug transporters
cholesterol ester transfer protein
CETP
cholesterol acyltransferase
LCAT
water insoluble drugs
dilauryl fluorescein
DLF
plasma
drug transport