Evaluation or the Gold-Plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler
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Moore, Melody Ann. Evaluation of the Gold-plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler. Master of Science (Forensic Genetics), May 2003, 49 pages, 9 figures, 3 tables, 2 appendices, 24 references. The GeneAmp PRC System 9700 Thermal Cycler has been introduced with interchangeable silver and gold-plated silver sample blocks. To validate the new gold-plated silver sample block on the System 9700, amplifications were performed on both the gold-plated silver and the previously validated silver sample blocks. PCR amplifications using the AmpFLSTR Profiler Plus ID COfiler, Identifiler and SGM Plus typing kits (Applied Biosystems, Forster City, CA) were performed. Electrophoretic characteristics such as allele concordance, peak heights, and peak height ratios were used to discern any differences in the amplification capabilities of the two sample blocks. The results demonstrate that the PCR reactions on both silver and gold-plated silver blocks are equivalent. In addition, the data obtained (allele cells, peak heights, peak height ratios) from both Macintosh and Windows NT platforms were identical for each amplification kit, indicating concordance between the software packages of each operating system. The results of this study demonstrate the interchangeability of the silver and gold-plated sample blocks.
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Equipment and Supplies
Genetic Processes
Genetics
Genetics and Genomics
Health Information Technology
Investigative Techniques
Life Sciences
Medical Genetics
Medicine and Health Sciences
Molecular Genetics
Other Genetics and Genomics
Gold-plated silver sample block
GeneAmp PCR System
9700 Thermal Cycler
interchangeable
amplifications
electrophoretic characteristics
allele concordance
peak heights
peak height ratio
software packages
operating systems
interchangeability
Macintosh
Windows NT platforms