Effect of estradiol replacement on hippocampal concentrations of estrogens in aged rhesus macaques maintained on an obesogenic diet

dc.creatorProkai, Laszlo
dc.creatorNguyen, Vien
dc.creatorUrbanski, Henryk F.
dc.creator.orcid0000-0002-4559-3458 (Prokai, Laszlo)
dc.date.accessioned2023-09-28T16:45:09Z
dc.date.available2023-09-28T16:45:09Z
dc.date.issued2023-09-25
dc.description.abstractReplacement involving estrogens has proven efficacy at treating a wide range of disorders that develop with menopause or after surgical removal of the ovaries. Here, we tested whether an estradiol (E2) replacement paradigm that recapitulates physiological E2 levels in the circulation also recapitulates physiological E2 levels within the hippocampus. E2 was delivered continuously to old ovariectomized (OVX) rhesus macaques, maintained on a high-fat, high-sugar Western-style diet (WSD) for approximately 30 months, via subcutaneous implants; this resulted in physiological concentrations of both estrone (E1) and E2 in the circulation (determined by LC-MS/MS). Surprisingly, however, hippocampal concentrations of E2 were markedly (P < 0.01) higher than in ovary-intact animals maintained on a regular chow diet. The data suggest that E2 replacement paradigms that appear to recapitulate physiological E2 concentrations in the circulation may produce hyper-physiological E2 levels within some brain areas, especially when individuals are maintained on a WSD.
dc.description.sponsorshipThis work was supported by National Institutes of Health Grants: P30 AG066518, P51 OD011092, R01 AG070072, R24 OD011895 and RF1 AG062220, and by The Welch Foundation's endowment BK-0031.
dc.identifier.citationProkai, L., Nguyen, V., & Urbanski, H. F. (2023). Effect of estradiol replacement on hippocampal concentrations of estrogens in aged rhesus macaques maintained on an obesogenic diet. Biochemistry and biophysics reports, 35, 101548. https://doi.org/10.1016/j.bbrep.2023.101548
dc.identifier.issn2405-5808
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32414
dc.identifier.volume35
dc.publisherElsevier B.V.
dc.relation.urihttps://doi.org/10.1016/j.bbrep.2023.101548
dc.rights.holder© 2023 The Authors.
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceBiochemistry and Biophysics Reports
dc.subjectestradiol
dc.subjectestrone
dc.subjectLC-MS/MS
dc.subjectrhesus macaque
dc.subjectWestern-style diet
dc.titleEffect of estradiol replacement on hippocampal concentrations of estrogens in aged rhesus macaques maintained on an obesogenic diet
dc.typeArticle
dc.type.materialtext

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