Variation in Mitochondrial DNA Heteroplasmy from Blood, Buccal, and Hair Samples

dc.contributor.advisorBudowle, Bruce
dc.contributor.committeeMemberGwirtz, Patricia A.
dc.contributor.committeeMemberCihlar, Jennifer C.
dc.creatorColon, Natalie S.
dc.date.accessioned2020-04-17T20:00:55Z
dc.date.available2020-04-17T20:00:55Z
dc.date.issued2020-05
dc.description.abstractMitochondrial DNA (mtDNA) sequence variation has important forensic implications. This research assesses five subjects for variation in point heteroplasmy (PHP) using blood, buccal, and head hair from five scalp sites. Five hairs from each site (25 total hairs) were sequenced for mtDNA using the Ion Chef and S5 technologies. Three hairs were used to study PHP variation along the hair shaft. Variance in hair PHP was more similar to blood (R2 = 0.3411) than buccal (R2 = 0.3059). Given sufficient signal, PHP was detected in all subjects, but was not concordant within or across all scalp sites. Along hair shafts, PHP was present in one distal hair portion. Future research is needed to elucidate PHP's impact on hair sample interpretation.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29833
dc.language.isoen
dc.subjecthair shaft
dc.subjectMitochondrial DNA
dc.subjectpoint heteroplasmy
dc.subject.meshDNA, Mitochondrial / genetics
dc.subject.meshForensic Genetics / methods
dc.subject.meshGenome, Mitochondrial / genetics
dc.subject.meshHair / chemistry
dc.subject.meshGenetic Variation
dc.titleVariation in Mitochondrial DNA Heteroplasmy from Blood, Buccal, and Hair Samples
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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