Effect of Detergent Selection on Quantity of DNA Obtained and on STR Profile Developed from Bone-Derived DNA

dc.contributor.advisorWarren, Joseph E.
dc.contributor.committeeMemberLaRue, Bobby L.
dc.contributor.committeeMemberKrishnamoorthy, Raghu R.
dc.creatorProctor, Frances N.
dc.date.accessioned2019-08-22T19:46:49Z
dc.date.available2019-08-22T19:46:49Z
dc.date.issued2016-05-01
dc.date.submitted2018-08-16T11:42:08-07:00
dc.description.abstractBone is sometimes the only source of DNA in cases of unidentified persons, missing persons, and mass fatality incidents, but bone characteristically provides lower quantities of DNA and lower quality short tandem repeat (STR) profiles than that of other sample types. Bone composition is very different from that of blood and soft tissue, therefore using a method designed for these other sample types is less effective for bone. A bone-specific extraction method is needed in order to improve these results. This study investigated the effects of detergent selection on both the quantity of DNA obtained and on the STR profiles produced from bone-derived DNA, as a part of developing a bone-specific DNA extraction protocol.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/26969
dc.language.isoen
dc.provenance.legacyDownloads8
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectForensic
dc.subjectbone
dc.subjectSTR
dc.subjectDNA
dc.titleEffect of Detergent Selection on Quantity of DNA Obtained and on STR Profile Developed from Bone-Derived DNA
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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