Validation of HemaSpotTM devices for the collection and long-term, room temperature storage of biological fluids from forensic reference samples

As forensic DNA samples spend more time in storage, collection devices capable of long-term, room temperature preservation provide cost-effective solutions. This project evaluated two different HemaSpot(TM) cartridges for collecting and storing human bodily fluids. Sensitivity was assessed by spotting five different volumes of blood and saliva from two individuals. Stability was examined by storing the sample collection membranes at 100 degrees C to simulate long-term storage. After 24 hours, DNA was extracted from eight 1.2-mm punches, quantified, amplified, and categorized by STR locus. Exposure to elevated temperature resulted in degradation based on quantification quality indicators and electropherogram results. Despite these observations, full STR profiles were obtained for the majority of sensitivity and stability samples. HemaSpotTM devices demonstrate potential in forensic applications for stabilizing reference DNA samples.