Validation of HemaSpotTM devices for the collection and long-term, room temperature storage of biological fluids from forensic reference samples

dc.contributor.advisorWarren, Joseph E.
dc.contributor.committeeMemberPlanz, John V.
dc.contributor.committeeMemberGaydosh-Combs, Laura
dc.contributor.committeeMemberMaddux, Scott D.
dc.creatorGonzalez, Kimberly A.
dc.date.accessioned2020-09-02T20:02:06Z
dc.date.available2020-09-02T20:02:06Z
dc.date.issued2019-05
dc.description.abstractAs forensic DNA samples spend more time in storage, collection devices capable of long-term, room temperature preservation provide cost-effective solutions. This project evaluated two different HemaSpot(TM) cartridges for collecting and storing human bodily fluids. Sensitivity was assessed by spotting five different volumes of blood and saliva from two individuals. Stability was examined by storing the sample collection membranes at 100 degrees C to simulate long-term storage. After 24 hours, DNA was extracted from eight 1.2-mm punches, quantified, amplified, and categorized by STR locus. Exposure to elevated temperature resulted in degradation based on quantification quality indicators and electropherogram results. Despite these observations, full STR profiles were obtained for the majority of sensitivity and stability samples. HemaSpotTM devices demonstrate potential in forensic applications for stabilizing reference DNA samples.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29853
dc.language.isoen
dc.subjectforensic science
dc.subjectvalidation
dc.subjectDNA
dc.subjectroom temperature
dc.subjectHemaSpot
dc.subject.meshPreservation, Biological
dc.subject.meshSpecimen Handling
dc.subject.meshForensic Sciences
dc.titleValidation of HemaSpotTM devices for the collection and long-term, room temperature storage of biological fluids from forensic reference samples
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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