INTERACTIONS BETWEEN T-CELLS AND ASTROCYTES POST-ISCHEMIC STROKE
dc.contributor.author | Yang, Shaohua | |
dc.creator | Hersh, Jessica | |
dc.date.accessioned | 2019-08-22T20:10:26Z | |
dc.date.available | 2019-08-22T20:10:26Z | |
dc.date.issued | 2018-03-14 | |
dc.date.submitted | 2018-02-20T18:13:48-08:00 | |
dc.description.abstract | Purpose: In post-ischemic stroke, T-lymphocytes enter the brain. Presently, the role of these T-cells in the progression of cerebral infarction or repair mechanisms is unclear. Our goal is to analyze the function of T-cells in regions of cerebral infarction by examining the pro- and anti-inflammatory interaction between T-cells and brain cells within these lesions. Methods: Ischemic stroke was induced by middle cerebral artery occlusion in young adult C57/B6 male mice. Mice were sacrificed at 3 days or 1-month post-ischemic stroke. T-cells were harvested from the brain by digestion; percoll enriched, and incubated with anti-CD3 and CD25 antibodies. T-cells were sorted via flow cytometry. The cytokine expression profile of brain infiltrated T-cells was compared to spleen T-cells using q-RTPCR. Results: In this in vivo study the following cytokines, poststroke, were found to be elevated: IFN-γ, IL-10, IL-17, TNF, Perforin, T-bet, and RORc. Conclusions: Our data suggests that understanding the interaction between T-cells and astrocytes could open new therapeutic strategies for stroke patients. | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/27973 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 0 | |
dc.title | INTERACTIONS BETWEEN T-CELLS AND ASTROCYTES POST-ISCHEMIC STROKE | |
dc.type | poster | |
dc.type.material | text |