Planz, John V.2020-04-172020-04-172020-05https://hdl.handle.net/20.500.12503/29836Internal validations are done in forensic laboratories to ensure the methods and procedures are working as expected. For this internship practicum, I conducted an internal validation of Promega's PowerPlex(R) Fusion 6C System, as well as, a Quarter Plate Method for the Hamilton MICROLAB(R) STARlet Robotic System. This validation project was done for the Kansas Bureau of Investigation's DNA Databank Unit. This validation consisted of testing a PBS wash versus no wash, a sensitivity study, an injection time study, an analytical threshold study, a stochastic threshold study, a precision study a reproducibility study, and a contamination study. The results of these validation studies demonstrated that both the novel Quarter Plate Method that was created and Promega's PowerPlex(R) Fusion 6C System can be successfully used by the DNA Databank Unit for sample processing.application/pdfenforensic STR loci/markersquarter plate methodreference DNA samplesDatabases, Nucleic Acid / standardsDatabases, Nucleic Acid / instrumentationForensic Genetics / instrumentationValidation StudyThesis