Arthur Eisenberg2019-08-222019-08-222002-08-012014-05-13https://hdl.handle.net/20.500.12503/29124Johnson, C., Validation of a Y-Chromosomal Short Tandem Repeat 10-Plex for Use in Forensic and Paternity Laboratories. Master of Science (Forensic Genetics), August, 2002. 94 pp., 6 tables, 7 figures, references, 21 titles. Y-chromosome microsatellite markers (Y-STRs) provide valuable information in cases of rape and questioned paternity, and they allow for the genetic identification of males. The present study validated a Y-STR 10-plex on the ABI Prism 3100 Genetic Analyzer for use in forensic and paternity laboratories at Orchid GeneScreen. Following optimization of the polymerase chain reaction, father-son pairs were analyzed to ensure that each pair generated identical haplotypes. The mutation rate varied between 0-0.238 (+/- 0.045, 95% confidence interval). The present study demonstrated that the 10-plex is sensitive to 0.75 ng and that female samples mixed with male samples did not interfere with Y-STR haplotyping. A population database of 525 males was developed and subsequently analyzed. Three instances of locus multiplication were observed, two at DYS19 and one at DYS435. Overall haplotype diversity was 0.996, suggesting that the 10-plex can efficiently distinguish male Y-STR profiles.application/pdfenCell and Developmental BiologyComputational BiologyEquipment and SuppliesForensic Science and TechnologyGeneticsGenetics and GenomicsGenetic StructuresHealth Information TechnologyInvestigative TechniquesLife SciencesMedicine and Health SciencesOther Genetics and GenomicsValidationy-chromosomal short tandem repeat10-plexforensic laboratoriespaternity laboratoriesrapeY-chromosome microsatellite markersY-STRsgenetic identificationmalesOrchid GeneScreenABI Prism 3100 Genetic Analyzermutation ratehaplotype diversitypopulation databaseProfessional Report