Walter McConathy2019-08-222019-08-222004-05-012014-02-24https://hdl.handle.net/20.500.12503/29414Johnson, Shemedia J., Horse Serum High Density Lipoproteins (HDL) as Drug Transporters. High-density lipoproteins (HDL) are complex particles composed of specific proteins and lipids that facilitate blood and tissue cholesterol homeostasis by transporting excess peripheral cholesterol to the liver. In association with cholesterol ester transfer protein (CETP) and the enzyme, lecithin: cholesterol acyltransferase (LCAT), HDL contributes to the transport of hydrophobic lipids, including cholesterol ester and triglycerides through the blood. The studies presented here involve the evaluation of horse serum HDL as a carrier of water insoluble drugs and an improved process to isolate and purify horse serum HDL utilizing hydrophobic affinity chromatography. Dilauryl fluorescein (DLF) has been chosen as a model compound for the study of horse HDL as a drug carrier. The prepared HDL/DLF particles have similar flotation densities and size properties to native horse serum HDL. The amount of DLF incorporated into HDL is 30μg/mg protein. Various cancer cell lines internalized DLF from horse HDL/DLF particles successfully. While human plasma contains cholesterol ester transfer protein (CETP), horse plasma does not. Horse plasma/serum can be supplemented by human plasma to study the role of CETP in drug transport and the stability of the horse HDL/drug complex.application/pdfenBiomechanics and BiotransportCell and Developmental BiologyChemicals and DrugsCirculatory and Respiratory PhysiologyHemic and Immune SystemsLife SciencesMedicinal Chemistry and PharmaceuticsMedicine and Health SciencesOther Pharmacy and Pharmaceutical SciencesPharmacologyPharmacy and Pharmaceutical SciencesHorse serum high density lipoproteinsHDLdrug transporterscholesterol ester transfer proteinCETPcholesterol acyltransferaseLCATwater insoluble drugsdilauryl fluoresceinDLFplasmadrug transportThesis