Simecka, Jerry W.2019-08-222019-08-222008-04-012014-04-01https://hdl.handle.net/20.500.12503/27835Spear, Marcia. The Impact of Mycoplasma pulmonis MALP-2 Homologue on Disease Progression. Master of Science (Biomedical Sciences), April 2008. 64 pp., 3 tables, 8 illustrations. Using Mycoplasma pulmonis, this project looked at a possible critical component in mycoplasma disease, the MALP-2 homologue lipoprotein. Studies demonstrated other lipoproteins besides the MALP-2 homologue were critical for in vivo disease progression and in vitro macrophage IL-6, IL-12, and TNF-α cytokine production. This trend was also seen human endothelial kidney (HEK) cells transfected with toll-like receptor 1 (TLR2) and the heterodimer TLR2/6. An increase in IL-8 cytokine production seen in all stimulated HEK cell lines, indicating the lipoproteins involved in cell interactions are TLR2 mediated. This project suggests the M. pulmonis MALP-2 homologue is not the main lipoprotein involved in disease progression and cell interactions, indicating the MALP-2 homologue may not be an ideal target for vaccines or antibiotics.application/pdfenBacteriaBacterial Infections and MycosesCell AnatomyCell and Developmental BiologyCell BiologyCellsCellular and Molecular PhysiologyDevelopmental BiologyDiseasesImmunology and Infectious DiseaseLife SciencesMedical Cell BiologyMedical MicrobiologyMedical Molecular BiologyMedicine and Health SciencesOther Cell and Developmental BiologyVirus DiseasesMycoplasma pulmonisMALP-2homologuedisease progressionimpactbiomedical sciencemycoplasma diseaselipoproteinin vivo disease progressionin vitro macrophageIL-6IL-12TNF-α cytokinehuman endothelial kidney cellsM. pulmoniscell interactionvaccinesantibioticsThesis