Coble, Michael D.2024-06-042024-06-042023-05https://hdl.handle.net/20.500.12503/32827Current methods for forensic DNA typing focus on the identification and processing of bodily fluids and tissues, while touch DNA evidence has remained less used due to the inability to detect where this type of DNA is deposited. This study utilizes four DNA-binding fluorescent dyes (Diamond™ Nucleic Acid Dye, SYBR™ Green I Nucleic Acid Stain, Ethidium Bromide, and YOYO™) to detect DNA at three concentrations (100, 500, and 1000 pg/μL) and explores the interactions and effects the dye may have on subsequent genetic profiling. Genetic profiling was conducted at three time points (0, 1, and 4 weeks,) via STR amplification and capillary electrophoresis (CE). DNA was detected using all dyes and increases in fluorescence were observed in response to increases in DNA concentration or dye volume added. Most samples produced full genetic profiles, all of which were stable over the 4-week time period.application/pdfenforensicstouch DNAgenetic profilingfluorescenceDNA detectionFluorescent DyesGenetic ProfileForensice GeneticsThesis