Arthur Eisenberg2019-08-222019-08-222003-05-012014-03-18https://hdl.handle.net/20.500.12503/29526Moore, Melody Ann. Evaluation of the Gold-plated Silver Sample Block on the GeneAmp PCR System 9700 Thermal Cycler. Master of Science (Forensic Genetics), May 2003, 49 pages, 9 figures, 3 tables, 2 appendices, 24 references. The GeneAmp PRC System 9700 Thermal Cycler has been introduced with interchangeable silver and gold-plated silver sample blocks. To validate the new gold-plated silver sample block on the System 9700, amplifications were performed on both the gold-plated silver and the previously validated silver sample blocks. PCR amplifications using the AmpFLSTR Profiler Plus ID COfiler, Identifiler and SGM Plus typing kits (Applied Biosystems, Forster City, CA) were performed. Electrophoretic characteristics such as allele concordance, peak heights, and peak height ratios were used to discern any differences in the amplification capabilities of the two sample blocks. The results demonstrate that the PCR reactions on both silver and gold-plated silver blocks are equivalent. In addition, the data obtained (allele cells, peak heights, peak height ratios) from both Macintosh and Windows NT platforms were identical for each amplification kit, indicating concordance between the software packages of each operating system. The results of this study demonstrate the interchangeability of the silver and gold-plated sample blocks.application/pdfenCell and Developmental BiologyEquipment and SuppliesGenetic ProcessesGeneticsGenetics and GenomicsHealth Information TechnologyInvestigative TechniquesLife SciencesMedical GeneticsMedicine and Health SciencesMolecular GeneticsOther Genetics and GenomicsGold-plated silver sample blockGeneAmp PCR System9700 Thermal Cyclerinterchangeableamplificationselectrophoretic characteristicsallele concordancepeak heightspeak height ratiosoftware packagesoperating systemsinterchangeabilityMacintoshWindows NT platformsProfessional Report