Attribution 4.0 International (CC BY 4.0)2022-07-112022-07-112018-08-01Mock, J. T., Knight, S. G., Vann, P. H., Wong, J. M., Davis, D. L., Forster, M. J., & Sumien, N. (2018). Gait Analyses in Mice: Effects of Age and Glutathione Deficiency. Aging and disease, 9(4), 634-646. https://doi.org/10.14336/AD.2017.09252152-5250https://hdl.handle.net/20.500.12503/31557Minor changes (~0.1 m/s) in human gait speed are predictive of various measures of decline and can be used to identify at-risk individuals prior to further decline. These associations are possible due to an abundance of human clinical research. However, age-related gait changes are not well defined in rodents, even though rodents are used as the primary pre-clinical model for many disease states as well as aging research. Our study investigated the usefulness of a novel automated system, the CatWalk XT, to measure age-related differences in gait. Furthermore, age-related functional declines have been associated with decreases in the reduced to oxidized glutathione ratio leading to a pro-oxidizing cellular shift. Therefore the secondary aim of this study was to determine whether chronic glutathione deficiency led to exacerbated age-associated impairments. Groups of male and female wild-type (gclm(+/+)) and knock-out (gclm(-/-)) mice aged 4, 10 and 17 months were tested on the CatWalk and gait measurements recorded. Similar age-related declines in all measures of gait were observed in both males and females, and chronic glutathione depletion was associated with some delays in age-related declines, which were further exacerbated. In conclusion, the CatWalk is a useful tool to assess gait changes with age, and further studies will be required to identify the potential compensating mechanisms underlying the effects observed with the chronic glutathione depletion.http://creativecommons.org/licenses/by/4.0/agingcatwalkgaitglutathione deficiencyspeedArticle© 2018 Mock et al.94