Agarwal, Neeraj2019-08-222019-08-222001-08-012014-04-09https://hdl.handle.net/20.500.12503/29068Vopat, K., Alterations in mRNA Levels of Selected Gene Products during Hypoglycemia, Hypoxia, and Ischemia Induced Apoptosis of Cultured Rat Retinal Ganglion Cells. Master of Science (Biomedical Science), August 2001. 54 pp., 2 tables, 10 illustrations, bibliography, 105 titles. In order to explore the mechanisms involved in the signal transduction pathways of ischemia-induced apoptosis of RGCs in glaucoma, an in vitro ischmia model of transformed rat retinal ganglion cells (RGC-5) was utilized. RGC-5 cells were exposed to hypoglycemia, hypoxia, and ischemia for six hours. Hypoxia and ischemia resulted in apoptosis of RGC-5 cells as determined by TUNEL assay. The bax mRNA levels increased significantly in cells exposed to hypoxia. The mRNA levels of hemoxygenase, c-fos HSP 70, and BDNF showed a trend of increase in both the hypoxic and ischemic conditions. These results demonstrate that retinal ganglion cells undergo apoptosis in hypoxic conditions likely via an increase in bax/bcl-2. The up-regulation of BDNF and some stress proteins may be part of a cellular rescue effort trying to overcome the damage created by hypoxic and ischemic stresses.application/pdfenCell AnatomyCell and Developmental BiologyCell BiologyCellsCellular and Molecular PhysiologyComparative and Laboratory Animal MedicineDevelopmental BiologyDiseasesGenetic ProcessesGenetics and GenomicsGenetic StructuresLife SciencesMedical Cell BiologyMedical GeneticsMedicine and Health SciencesOther Cell and Developmental BiologyOther Genetics and GenomicsVision ScienceAlterationsmRNAselected gene productshypoglycemiahypoxiaischemiaapoptosiscultured rat retinal ganglion cellssignal transduction pathwaysglaucomacell apoptosishypoxicischemic conditionsBDNFstress proteinscellular rescue effortsThesis