Browsing by Subject "Bone"
Now showing 1 - 3 of 3
- Results Per Page
- Sort Options
Item Effects of Amicon Ultra-4 Centrifugal Devices on DNA Yield From Bone Samples and Effects of Amplicon Rx Post-PCR Treatment onRelative Florescence Units Obtained During Capillary Electrophoresis(2014-05-01) Amiel, Marie B.; Arthur EisenbergThe ability to recover DNA and perform DNA analysis from skeletal remains is a valuable tool for identification of missing persons and unidentified remains. DNA extraction from bone often yields low levels of DNA, which can inhibit analysis and impact genetic profiles. Efficient recovery of DNA from bones is therefore vital. In this study, three molecular weight cut-off columns were evaluated. The Amicon® 10,000 NMWL filter device proved to be most efficient at retaining amplifiable DNA and obtaining optimal genetic analysis results. Additionally, the ability of Amplicon RxTM, a post-PCR treatment, to improve genetic profiles by providing a boost in RFUs obtained during capillary electrophoresis was evaluated.Item Effects of pH on Binding of DNA Using the PrepFiler BTA Forensic DNA Extraction Kit on the AutoMate Express Forensic DNA Extraction System(2014-05-01) Ferguson, Erin E.; Arthur EisenbergThe extraction method for the recovery of DNA from bone samples at the UNT Center for Human Identification (UNTCHI) Missing Persons Laboratory is both time consuming and laborious. The results of this study show that the AutoMate Express™ can yield both DNA quantity and STR profiles comparable or greater to that of the standard organic extraction method. The incorporation of this automated technology could significantly reduce the time and streamline the process while increasing the amount of genetic information obtained. Based on the results of this study, the use of the AutoMate Express™ for the extraction of DNA from skeletal remains could be very advantageous to UNTCHI DNA analysts.Item Identification of Unknown PCR Products Generated During STR Analysis of Bone Samples(2013-05-01) Koehn, Alyssa R; Bruce BudowleSTR typing of DNA extracted from bone samples exhibited additional amplified products with the PowerPlex® 16 HS Kit (Promega Corp., Madison, WI) and the Identifiler® Plus Amplification kit (Life Technologies, Foster City, CA). Microbial DNA found in soil that is co-extracted and amplified with the human DNA from bones may be the source of the artifacts seen during the STR analysis of the DNA samples. This project tested the hypothesis that the additional PCR products found in the STR analysis of DNA derived from bone samples are microbial or fungal in origin. This study has demonstrated that amplification of soil samples with an STR kit can produce artifacts, similar to those seen in the bone samples. A protocol was developed to isolate the artifact products. Attempts were made to Sequence the amplified PCR product; however, no sequencing results have been obtained, further experiments continue to attempt to generate sequencing results.