Browsing by Subject "DNA / isolation & purification"
Now showing 1 - 2 of 2
- Results Per Page
- Sort Options
Item Alternative procedure for DNA recovery from cartridges and casings(2020-05) Bettex, Kelsey D.; Planz, John V.; Jones, Harlan P.; Warren, Joseph E.Both unfired and fired ammunition recovered from a crime scene are useful in connecting a crime to a specific firearm, however its use in identifying individuals is less common due to low-level or degraded DNA present. Traditionally, the standard procedure for DNA sampling of ammunition evidence is the use of a swabbing technique. The New York State Police (NYSP) Forensic Investigation Center conducted a study to test the efficacy of an alternative method for obtaining DNA from cartridges and casings, soaking the sample prior to extraction. This method presented a 186% increase in total DNA yield, 169% increase in male DNA yield, and a 44% increase in comparable profiles obtained using GlobalFiler(TM) and 240% using Yfiler(R) Plus. Results showed fewer 'no data' determinations using the soaking method, indicating more DNA obtained. Although a statistically significant difference was not observed between the two methods (p = 0.070), a qualitative significance was.Item The Validation of the RapidHIT ID System for Human Identification(2020-05) Campos, Gemma R.; Budowle, Bruce; Cihlar, Jennifer C.; Gwirtz, Patricia A.Forensic scientists routinely employ short tandem repeat (STR) typing as a means to identify or exclude individuals as the donors of biological evidence at crime scenes. Despite developments in automation of STR typing workflow, this time-consuming work requires the expertise and resources of a dedicated forensic genetics laboratory. Rapid DNA technology, a fully automated, turnkey system, offers a simple, 90-minute swab-to-profile approach to generate STR profiles, requiring minimal training and expertise to operate the system. As Rapid DNA continues to be developed and implemented, validation studies are necessary to ensure the quality of analyses performed by Rapid DNA instruments. A series of studies in accordance with SWGDAM guidelines were designed to test different performance measures of the RapidHIT ID System for Human Identification (Thermo Fisher Scientific). The results from this study provide opportunities for protocol and user guide revisions, software improvements, and highlight parts of instrumentation that can be improved for future models.