Browsing by Subject "IL-6"
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Item Characterization of IL-17A-Secreting CD8 T Cells(2008-12-01) Lee, Suheung; Harlan Jones; Porunellor Mathew; Peter KoulenIL-17A-secreting CD4 T cells (Th17 cells) have been demonstrated to play pivotal roles in modulating immune responses during various types to infectious and autoimmune diseases. While 1L-17A secreting CD8 T cells have been detected in numerous disease models, much less is known about them. In this thesis, the differentiation conditions and effector functions of IL-17A-secreting CD8 T cells have been examined. In order to differentiate naïve CD8 T cells into IL-17A secretors, TGF-β, IL-6, and neutralization of IFN-γ are required as in Th17 cells. IL-17A-secreting CD8 T cells produce the effector cytokines, IL-17A, IL-17F and IL-22, but do not produce granzyme B, implicating the lack of cytotoxicity. Furthermore, IL-17A-secreting CD8 T cells can respond to exogenous cytokines without a cognate antigen, suggesting that they can act in an innate fashion. Collectively, IL-17A-secreting CD8 T cells possess the same effector functions as Th17 cells, and thus may play as significant roles in various diseases at Th17 cells.Item The impact of early life stressors on the progression of SLE(2021-08) Hartman, Rusty L.; Mathis, Keisa W.; Cunningham, J. Thomas; Hodge, Lisa M.; Tune, Johnathan D.Our preliminary studies show that an established model of systemic lupus erythematosus (SLE), the female NZBWF1 mouse, had worsened indices of disease later in life when the mice were shipped to our institution at an early age during the summer. We hypothesized that interleukin (IL)-6-induced release of heat shock protein 90 (HSP90) is upregulated in response to this summer early-life stressor, thus accelerating autoimmunity and renal disease in female SLE mice. To begin to study this, we measured renal IL-6 and HSP90 in 6-week-old female NZBWF1 mice that were shipped in winter or summer months and found that both were elevated immediately following summer compared to winter travel. Our findings indicate that the mediators associated with early-life travel/seasonal stressors may predict the progression of autoimmunity in SLE-prone mice. Other findings here within highlight the specificity of this effect in the kidney and describe sex differences in the observed phenomena.Item The Impact of the Mycoplasma pulmonis MALP-2 Homologue on Disease Progression(2008-04-01) Spear, Marcia G.; Simecka, Jerry W.; Hodge, Lisa M.; Mathew, Porunelloor A.Spear, Marcia. The Impact of Mycoplasma pulmonis MALP-2 Homologue on Disease Progression. Master of Science (Biomedical Sciences), April 2008. 64 pp., 3 tables, 8 illustrations. Using Mycoplasma pulmonis, this project looked at a possible critical component in mycoplasma disease, the MALP-2 homologue lipoprotein. Studies demonstrated other lipoproteins besides the MALP-2 homologue were critical for in vivo disease progression and in vitro macrophage IL-6, IL-12, and TNF-α cytokine production. This trend was also seen human endothelial kidney (HEK) cells transfected with toll-like receptor 1 (TLR2) and the heterodimer TLR2/6. An increase in IL-8 cytokine production seen in all stimulated HEK cell lines, indicating the lipoproteins involved in cell interactions are TLR2 mediated. This project suggests the M. pulmonis MALP-2 homologue is not the main lipoprotein involved in disease progression and cell interactions, indicating the MALP-2 homologue may not be an ideal target for vaccines or antibiotics.