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Item Assessment and Identification of Areas for Improvement of a Local Health Department Food Safety Program(2008-05-01) Harris, Ann MarieHarris, Ann Marie. Assessment and Identification of Areas for Improvement of a Local Health Department Food Safety Program. Master of Public Health (Environmental Health), May 2008, 14 pp. 1 table, 1 figure, references, 14 titles. The Fort Worth Public Health Department (FWPHD) established a standardized assessment to compare compliance rates for risk factors contributing to foodborne illness. The FWPHD identified significantly higher compliance rates in four out of six risk factors. Risk factors posing the greatest risk for out of compliance observations included threats from contaminated equipment and chemical/other hazards. Fast food establishments had a significantly greater risk for contaminated equipment (OR=1.81; CI=1.27, 2.58). Chemical/other hazards was the only risk factor with a higher overall out of compliance rate than the FDA. The FWPHD can now accurately track the effectiveness of training and education programs for food handlers, consumer health specialists, and the overall inspection process.Item Fecal Coliforms in the Rio Grande: A Risk to Human Health(2004-12-01) Tompkins, Erin L.; Thomas Vaughan; Claudia S. CogginTompkins, Erin L., Fecal Coliforms in the Rio Grande: A Risk to Human Health. Master of Public Health (Environmental Health), December 2004, 45 pages, bibliography, 33 titles. The Rio Grande around Laredo, Texas/Nuevo Laredo, Mexico was designated for primary contact reaction by the EPA. However, monthly sampling over a ten-year period in this section of the river may show otherwise. Fecal contamination of the Rio Grande in this area may be a source of illness to the population. Four sites in Laredo area were tested for fecal coliform density and rate of flow. Rainfall data from the USGS was used for comparisons. The rate of flow of the Rio Grande had an impact on fecal coliform density at one site measured. Rainfall in Laredo had an impact on fecal coliform density at two measured sites, and was a significant predictor of density at these sites as well. A review of the designation for this river segment is recommended. More research is needed to determine the exposed population, and effects of high coliform densities on downstream communities.Item Predictors of Complicated Staphylococcus Aureus Bacteremia: A Retrospective Validation Study(2008-04-01) Krishnamurthy, Pramod; Fischbach, Lori; Cardarelli, Roberto; Coggin, Claudia S.Krishnamurthy, P., Predictors of Complicated Staphylococcus aureus Bacteremia (SAB): A Retrospective Validation Study. Master of Public Health (Epidemiology), April 2008, 57 pp, 9 tables, 1 illustration, bibliography, 39 titles. SAB often has a complicated clinical course and it is important to identify those at risk for complications to guide management. We conducted a validation study of a clinical prediction tool that uses a scoring system to predict the likelihood of developing complicated SAB. Chapter I is a review of background literature and rationale for our study. Chapter II has sections describing the study design, methods, eligibility criteria, statistical analysis and a summary of the results. We observed significantly higher complications among patients with SAB in our validation study. The prediction tool is not a valid predictor of complicated SAB and we recommend better prediction models to accurately predict complications of SAB.Item Resistance of Bacillus Subtilis Spores Lacking Either Nucleotide Excision Repair or Spore Photoproduct Lyase to Ultraviolet (UV) Radiation from Artificial or Natural Sources(1996-06-01) Xue, Yaming; Tony Romeo; Ming-Chi Wu; Wayne L. NicholsonXue, Yaming, Resistance of Bacillus subtilis spores lacking either nucleotide excision repair or spore photoproduct lyase to ultraviolet (UV) radiation from artificial or natural sources. Master of Science (Biomedical Sciences), June 1996, pp., 4 tables, 12 illustrations, references, 38 titles. Exposure of bacterial spores to UV radiation causes the accumulation of a unique pyrimidine dimer in the DNA, “spore photoproduct” (SP). In Bacillus subtilis, two distinct DNA repair pathways are used for removal of SP: general nucleotide excision repair (the uvr parthway), or the SP-specific enzyme SP lyase (the spl pathway). Spores of four strains of Bacillus subtilis differing in their repair capabilities were irradiated under either artificial or solar UV. To determine the biologically-relevant cumulative UV dose under each irradiation condition, a sporocidal dosimeter was constructed. The results showed: (i) Both uvr and spl pathways contributed to the survival of spores under all tested conditions. The spl pathway was more efficient than uvr pathway in repairing the DNA damage caused by UV-C and solar UV-A, but no significant difference was noted in repairing DNA damage caused by UV-B or full-spectrum solar UV. (ii) Exposure of spores to solar UV can cause cellular lethal damage which is reparable by neither repair pathway.