Browsing by Subject "Population Biology"
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Item Allele Characterization of Fifteen Short Tandem Repeat Loci of North American Golden (Aquila Chysaetos) and Bald (Haliaeetus leucocephalus) Eagles using Next-Generation Sequencing(2014-05-01) Howard, Taylor E.; John PlanzBald and golden eagles are species of conservation concern in North America, and are protected under the Bald and Golden Eagle Protection Act (BGEPA; 16 U.S.C. 668-668d). Wildlife forensics utilizes short tandem repeat (STR) loci for identification purposes, however the loci currently used for bald and golden eagle in North America were developed from related species of European eagles. In this study, STR loci were sequenced using the Ion Torrent™ Personal Genome Machine (PGM™) Sequencer® (Life Technologies™, Carlsbad, CA) to characterize the alleles (e.g. the repeat motifs, presence of SNPs and indels). These methods were used to evaluate the discriminatory power of the loci for individualization and for species differentiation.Item Genetic Diversity of Easter Island (Rapanui) Population from Identifiler® Plus autosomal, Y-filer®, and Y-Plex™ 6 Y-STR Loci(2015-05-01) Guadian, Laura; Chakraborty, Ranajit; Budowle, Bruce; Hodge, Lisa M.This study investigated the genetic diversity of the Easter Island (Rapanui) population using data on 15 autosomal Short Tandem Repeats (STRs) typed with the commercial STR kits Identifiler® Plus and 23 Y-chromosome STRs typed using Y-filer (17 loci) and Y- PLEX™ 6 (6 loci). The analysis was conducted using genotype and haplotype data of 122 presumably unrelated individuals that included 48 males and 74 females. This study: (i) examined if Easter Island population had reduced genetic diversity in comparison with cosmopolitan populations such as Mainland Chilean, Polynesian, European, and African; (ii) compared genetic affinity of the Easter Island population with historically related cosmopolitan populations; and (iii) investigated the forensic utility of autosomal STRs and Y-STRs in the Easter Island population.Item The Population Abundance and Associated Geographic and Demographic Factors of the Dengue Vectors, Aedes Aegypti and Aedes Albopictus in Dallas County, TX, USA(2007-05-01) Stahl, Matthew S.; Sue Lurie; Joon-Hak LeeStahl, Matthew S., The Population Abundance and Associated Geographic and Demographic factors of the Dengue Vectors Aedes aegypti and Aedes albopictus in Dallas County, TX. Master of Public Health (Environmental Health), May 2007, 40 pp., 7 figures, 2 tables, bibliography, 72 titles. The risk for dengue outbreak was assessed in North Central Texas in 2006 in response to increased case numbers in Texas and Mexican states in 2005. Data were collected from 54 sites in Dallas County, TX using oviposition traps and estimates from U.S. Census and Sourcebook America databases. Higher vegetation and shade displayed more Aedes species; standing water also showed more Aedes albopictus. Lower home values and lower incomes corresponded to more Aedes aegypti; lower household density displayed more Aedes albopictus. Other socio-economic and demographic factors did not have significant association with abundance. The methodology of this study may serve as a model for assessment of dengue vector abundance in other regions.