Browsing by Subject "Skeletal remains"
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Item A Semi-Automated Methodology for Extraction of DNA from Human Skeletal Remains(2013-05-01) Mize, Mary L.; Arthur EisenbergThe current methodology used by the University of North Texas Center for Human Identification Missing Persons Laboratory (UNTCHI) to recover DNA from skeletal remains is time-consuming, laborious and not readily amenable to automation. The constraints of the current process limit the number of samples that can be analyzed. The results of this study show that extractions performed with the AutoMate Express™ Forensic DNA Extraction System (Life Technologies, Carlsbad, CA) can produce comparable DNA quantity and quality to the current procedure used by UNTCHI. The utilization of the AutoMate Express™ Forensic DNA Extraction System in our operational laboratories would help streamline the process of DNA extraction from human skeletal remains and potentially provide increased amounts of genetic information.Item Typing Success of Short Tandem Repeat Loci on Skeletal Remains: Comparison of the AmpFLSTR® Identifiler® Plus and GlobalFiler™ PCR Amplification Kit.(2014-05-01) Garcia, Stephanie A.; Arthur EisenbergThe AmpFLSTR® Identifiler® Plus PCR Amplification Kit (Life Technologies, Carlsbad, CA), and the recently released GlobalFiler™ PCR Amplification Kit (Life Technologies) were compared to determine which provided the greater power of discrimination from DNA extracted from skeletal remains. I hypothesized, STR profiles obtained using the GlobalFiler™ kit for analysis of skeletal remains would result in an increased number of reportable genetic loci, and provide greater power of discrimination as compared to the Identifiler® Plus Kit. The results of this study showed GlobalFiler™, along with an automated extraction, could produce comparable or greater genetic typing results and discriminatory power from skeletal remains without the use of a second amplification kit.