Browsing by Subject "Touch DNA"
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Item Assessment of DNA transfer Involving Routine Human Behavior(2010-05-01) Kelley, Shamika; Bruce BudowleDNA transfer events are affected by routine human actions and can impact the interpretation of forensic evidence results. Some scientists have inferred that secondary transfer events lead to only minimal amount of DNA yield and when two people are involved, the DNA profile of the primary person who had contact with the item is typically prominent. To assess the effects of secondary DNA transfer events on DNA quantity, methods similar to those of Lowe et al. [1] were used. We have recruited 12 volunteers (subjects) to participate in a 4-part study consisting of every day human routine behaviors. These routine behaviors include handshaking, holding a pen in the mouth, and licking the thumb before turning the page. Sufficient quantities of DNA were obtained via secondary and tertiary transfer. DNA profiles could be observed from an individual to an object even though that individual did not directly touch the object.Item Validation of the ParaDNA[R] Screening System with touch DNA using mock evidence samples(2018-05) West, Whitney R.; Allen, Michael S.; Warren, Joseph E.; Staub, Rick W.; Schreihofer, Ann M.Short tandem repeat, or STR, analysis is expensive and often creates a waiting game for law enforcement agencies to receive these results due to high demand and the current backlog at forensic laboratories. ParaDNA[R] by LGC utilizes HyBeacon Probe technology to rapidly analyze DNA and provide a percentage value of the amount of DNA present, as well as 2-5 loci, depending on whether the Screening System or Intelligence System is being used. Currently, this technology is novel and is intended for sample screening and prioritizing purposes for the Plano Police Department. This validation study has shown that the ParaDNA[R] Screening System can obtain genetic data from touch DNA and trace DNA samples by using both direct and indirect sampling methods. The results from this validation have further indicated that if the Screening System provides a score higher than 60%, these samples should be prioritized and sent to forensic laboratories for full STR analysis.