Browsing by Subject "Validation Studies"
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Item A Bone and Buccal Sensitivity Study Comparison and Stability Study using the PowerPlex[R] Fusion 6C System(2018-05) McDaniel, Ethan L.; Warren, Joseph E.; Planz, John V.; Krishnamoorthy, Raghu R.; Gaydosh-Combs, LauraA validation study, a bone sensitivity study and a stability study were performed using the PowerPlex[R] Fusion 6C System. These studies were performed on a 7500 Real-Time PCR System, 9700 GeneAmp Thermocycler and 3500xL Genetic Analyzer. Buccal DNA was used to develop a method to analyze the DNA profiles gathered during the bone sensitivity study and stability study. DNA profiles for specific concentrations of DNA in solution were obtained during the bone sensitivity study. The stability study showed profiles exhibiting the effects of metal PCR inhibitors being introduced to the DNA extract solutions. Full profiles were obtained for calcium concentrations less than 7.35 mM, while the instrument was fully inhibited for copper concentrations between 0mM and 7.35 mM. Based on the limited data, the PowerPlex Fusion 6C System cannot tolerate copper when present in DNA solutions; whereas, calcium may be tolerated as an inhibitor up to 7.35mM.Item ParaDNA: A Novel Instrument for Presumptive DNA Analysis(2018-05) Moussa, Ola; Warren, Joseph E.; Allen, Michael S.; Staub, Rick W.; Millar, J. CameronCurrent methods for human identification are time consuming and can take weeks to complete, leading to a backlog of evidence needing to be processed and a slowdown in investigations. The ParaDNA[R] Instrument is designed to address this issue using the Screening and Intelligence Systems which can detect the relative amount of DNA in an evidence item, and analyze 5 short tandem repeats (STRs) and Amelogenin, respectively [3]. The Instrument uses HyBeacons[R] which target specific STRs to identify the presence of DNA, and detect STR alleles. This is a validation of the ParaDNA[R] Screening and Intelligence Systems using saliva and blood samples to assess the sensitivity and reliability of the instrument. The data collected using the ParaDNA[R] Instrument show that it can reliably identify the relative amount of DNA in a sample, and display useful STR profiles that are 99% concordant with Qiagen Investigator 24Plex QS STR Kits.Item Validation of the ParaDNA[R] Screening System with touch DNA using mock evidence samples(2018-05) West, Whitney R.; Allen, Michael S.; Warren, Joseph E.; Staub, Rick W.; Schreihofer, Ann M.Short tandem repeat, or STR, analysis is expensive and often creates a waiting game for law enforcement agencies to receive these results due to high demand and the current backlog at forensic laboratories. ParaDNA[R] by LGC utilizes HyBeacon Probe technology to rapidly analyze DNA and provide a percentage value of the amount of DNA present, as well as 2-5 loci, depending on whether the Screening System or Intelligence System is being used. Currently, this technology is novel and is intended for sample screening and prioritizing purposes for the Plano Police Department. This validation study has shown that the ParaDNA[R] Screening System can obtain genetic data from touch DNA and trace DNA samples by using both direct and indirect sampling methods. The results from this validation have further indicated that if the Screening System provides a score higher than 60%, these samples should be prioritized and sent to forensic laboratories for full STR analysis.