Browsing by Subject "Validation Study"
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Item The Validation of the RapidHIT ID System for Human Identification(2020-05) Campos, Gemma R.; Budowle, Bruce; Cihlar, Jennifer C.; Gwirtz, Patricia A.Forensic scientists routinely employ short tandem repeat (STR) typing as a means to identify or exclude individuals as the donors of biological evidence at crime scenes. Despite developments in automation of STR typing workflow, this time-consuming work requires the expertise and resources of a dedicated forensic genetics laboratory. Rapid DNA technology, a fully automated, turnkey system, offers a simple, 90-minute swab-to-profile approach to generate STR profiles, requiring minimal training and expertise to operate the system. As Rapid DNA continues to be developed and implemented, validation studies are necessary to ensure the quality of analyses performed by Rapid DNA instruments. A series of studies in accordance with SWGDAM guidelines were designed to test different performance measures of the RapidHIT ID System for Human Identification (Thermo Fisher Scientific). The results from this study provide opportunities for protocol and user guide revisions, software improvements, and highlight parts of instrumentation that can be improved for future models.Item Validation of the PowerPlex(R) Fusion 6C System and Alternate Robotic Methods on the Hamilton MICROLAB(R) STAR(2020-05) Smith, Briana N.; Planz, John V.; Barber, Robert C.; Zascavage, Roxanne R.Internal validations are done in forensic laboratories to ensure the methods and procedures are working as expected. For this internship practicum, I conducted an internal validation of Promega's PowerPlex(R) Fusion 6C System, as well as, a Quarter Plate Method for the Hamilton MICROLAB(R) STARlet Robotic System. This validation project was done for the Kansas Bureau of Investigation's DNA Databank Unit. This validation consisted of testing a PBS wash versus no wash, a sensitivity study, an injection time study, an analytical threshold study, a stochastic threshold study, a precision study a reproducibility study, and a contamination study. The results of these validation studies demonstrated that both the novel Quarter Plate Method that was created and Promega's PowerPlex(R) Fusion 6C System can be successfully used by the DNA Databank Unit for sample processing.