Browsing by Subject "diabetes mellitus"
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Item Analysis of patient compliance and progression of diabetic foot ulcer healing(2017-08-01) Hussain, Waasae M.; Patricia A. Gwirtz; Stephen O. Mathew; Raghu R. KrishnamoorthySelf-reporting serves as a mask for non-compliance in subjects in research studies. If a subject stays compliant with research guidelines it is assumed their diabetic foot ulcer would heal. This study evaluates the correlation between alleged patient compliance and the rate of healing of diabetic foot ulcers using a retrospective chart analysis. There was no correlation seen and the results showed normal distribution. This could be caused by unreported non-adherence.Item Chronic Inhibition of Mitochondrial Dihydrolipoamide Dehydrogenase (DLDH) as an Approach to Managing Diabetic Oxidative Stress(MDPI, 2019-02-02) Yang, Xiaojuan; Song, Jing; Yan, Liang-JunMitochondrial dihydrolipoamide dehydrogenase (DLDH) is a redox enzyme involved in decarboxylation of pyruvate to form acetyl-CoA during the cascade of glucose metabolism and mitochondrial adenine triphosphate (ATP) production. Depending on physiological or pathophysiological conditions, DLDH can either enhance or attenuate the production of reactive oxygen species (ROS) and reactive nitrogen species. Recent research in our laboratory has demonstrated that inhibition of DLDH induced antioxidative responses and could serve as a protective approach against oxidative stress in stroke injury. In this perspective article, we postulated that chronic inhibition of DLDH could also attenuate oxidative stress in type 2 diabetes. We discussed DLDH-involving mitochondrial metabolic pathways and metabolic intermediates that could accumulate upon DLDH inhibition and their corresponding roles in abrogating oxidative stress in diabetes. We also discussed a couple of DLDH inhibitors that could be tested in animal models of type 2 diabetes. It is our belief that DLDH inhibition could be a novel approach to fighting type 2 diabetes.Item Deltoid Opioid Receptor Phenotype Modulation of Hindlimb Vascular Conduction(2008-10-06) Barlow, Matthew A.; Raven, Peter B.; Gwirtz, Patricia A.; Dillon, Glenn H.Barlow, Matthew A. Deltoid Opioid Receptor Phenotype Modulation of Hindlimb Vascular Conduction. Doctor of Philosophy (Integrative Physiology), Oct 6th, 2008, 136 pp, 1 table 26 figures. Hypertension, diabetes mellitus and their presumed precursor the metabolic syndrome are part of a complex disease process associated with insulin resistance. Neurovascular complications in diabetics commonly involve the lower limbs resulting in a vicious cycle of autonomic neuropathy, painful occlusive claudication and resulting immobility that precipitates inactivity and progressive disability. The fixed neural and vascular diseases evolve slowly and the early events in this progressive decline in function are poorly understood. Sympathetic vasoconstriction is a major component of blood flow regulation in muscle. Active vasoconstriction in the lower limbs depends on continued transmission of efferent vasomotor signals through the lumbar sympathetic chain ganglia. Opioid receptors actively reduce normal ganglionic transmission presumably by lowering acetylcholine release. In the heart, the subtypes of delta-opioid receptors (DORs) facilitate (DOR-1, vagotonic) and inhibit (DOR-2, vagolytic) cholinergic transmission in the heart. The DOR-2 mediated inhibitory effects in heart are alterable and can change rapidly. Diabetes impairs vascular control. Ganglionic transmission is metabolically vulnerable during high fat feeding and insulin resistance. We hypothesized that the DOR-2 stimulation significantly facilitates vasodilation by reducing cholinergic transmission within the sympathetic chain ganglion. The ability to activate DOR-1 stimulation facilitates to cause further vasoconstriction in the anesthetized and surgically instrumented state of the dog did not show dose dependent activation. The DOR-1 activity in the insulin resistant dogs appears to be decreased as the DOR-1 blockade had no effect on the dose responses in the heart or the hindlimb. Enhanced sympathetic tone through BCO by increasing and reducing cholinergic transmission in the lumbar sympathetic ganglion shows an enhanced pro-constrictor phenotype under stresses of severe hypotension possibly through a DOR-1 mediated activation.Item Meta-analysis: Effects of Opuntia Species(2000-05-01) Garcia, Anna R.; John Licciardone; Gilbert Ramirez; FongGarcia, Anna R., Meta-analysis: Effects of Opuntia species. Master of Public Health (Epidemiology), May, 2000, 73 pp., 10 tables, 10 figures, references, 62 titles. The Mexican American population is more susceptible to diabetes mellitus due to a number of risk factors. The earliest recorded treatments for diabetes mellitus involved the use of natural plants. Opuntia species are any member of the genus Opuntia of Cactus family and who are native to the Western Hemisphere. In order to determine the efficacy of Opuntia species as a hypoglycemic agent in non-insulin dependent diabetics, a meta-analysis was conducted to analyze the identified studies. In addition, insulin and the presence of a dose-response relationship upon ingestion of Opuntia were investigated. A statistically significant reduction in serum glucose was found after the ingestion of 500 grams of Opuntia species. Additional studies are needed to determine the mechanism of hypoglycemic action and to further investigate the properties of Opuntia species.Item Redox imbalance stress in diabetes mellitus: Role of the polyol pathway(John Wiley & Sons Australia, Ltd, 2018-04-19) Yan, Liang-JunIn diabetes mellitus, the polyol pathway is highly active and consumes approximately 30% glucose in the body. This pathway contains 2 reactions catalyzed by aldose reductase (AR) and sorbitol dehydrogenase, respectively. AR reduces glucose to sorbitol at the expense of NADPH, while sorbitol dehydrogenase converts sorbitol to fructose at the expense of NAD(+), leading to NADH production. Consumption of NADPH, accumulation of sorbitol, and generation of fructose and NADH have all been implicated in the pathogenesis of diabetes and its complications. In this review, the roles of this pathway in NADH/NAD(+) redox imbalance stress and oxidative stress in diabetes are highlighted. A potential intervention using nicotinamide riboside to restore redox balance as an approach to fighting diabetes is also discussed.Item Unravelling Novel Roles of Salivary Exosomes in the Regulation of Human Corneal Stromal Cell Migration and Wound Healing(MDPI, 2022-04-14) Escandon, Paulina; Liu, Angela; Nicholas, Sarah E.; Khan, Asher; Riaz, Kamran M.; Karamichos, DimitriosSalivary exosomes have demonstrated vast therapeutic and diagnostic potential in numerous diseases. This study pioneers previously unexplored roles of SE in the context of corneal wound healing by utilizing primary corneal stromal cells from healthy (HCFs), type I diabetes mellitus (T1DMs), type II DM (T2DMs), and keratoconus (HKCs) subjects. Purified, healthy human SEs carrying tetraspanins CD9+, CD63+, and CD81+ were utilized. Scratch and cell migration assays were performed after 0, 6, 12, 24, and 48 h following SE stimulation (5 and 25 microg/mL). Significantly slower wound closure was observed at 6 and 12 h in HCFs with 5 mug/mL SE and T1DMs with 5 and 25 mug/mL SE. All wounds were closed by 24-hour, post-wounding. HKCs, T1DMs, and T2DMs with 25microg/mL SE exhibited a significant upregulation of cleaved vimentin compared to controls. Thrombospondin 1 was significantly upregulated in HCFs, HKCs, and T2DMs with 25 microg/mL SE. Lastly, HKCs, T1DMs, and T2DMs exhibited a significant downregulation of fibronectin with 25 mug/mL SE. Whether SEs can be utilized to clinical settings in restoring corneal defects is unknown. This is the first-ever study exploring the role of SEs in corneal wound healing. While the sample size was small, results are highly novel and provide a strong foundation for future studies.