Browsing by Subject "lymphatic pump"
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Item Lymphatic Pump Treatment Enhances the Lymphatic and Immune System and Ameliorates Disease Severity in a Rat Model of Respiratory Infection(2014-05-01) Schander, Artur; Hodge, Lisa M.The purpose of these studies was to explore the benefits, effects, and mechanisms of LPT in both a healthy and a diseased animal model, and hence provide scientific rationale for the clinical application of LPT. Novel findings in this dissertation demonstrate that in anesthetized canines: 1) LPT mobilizes leukocytes from the GALT into lymphatic circulation; 2) LPT mobilizes inflammatory mediators into lymphatic circulation; and 3) repeated application of LPT increases lymph flow, concentration of leukocytes, and flux of inflammatory mediators into lymphatic circulation. In addition, this dissertation for the first time demonstrates: 1) the development of a novel lymph enhancing rodent model, in which LPT increases leukocyte flux in the cisterna chili, predominantly from the GALT; and 2) that LPT facilitates the clearance of pneumococcal respiratory infection and suggests a mechanism by which LPT might facilitate the clearance of pneumococcal pneumonia. Our studies demonstrated that LPT transiently mobilized leukocytes from the mesenteric lymph nodes. We found a significant increase in the concentrations of MCP-1 and flux of IL-6 flux in TDL and MDL in anesthetized dogs. Interestingly, both IL-6 and MCP-1 were present in BALF of rats infected with pneumococcus, and LPT significantly increased IL-6 and moderately increased MCP-1 concentrations compared to Sham and Control animals, which supports our notion that LPT may increase cytokine/chemokine redistribution from the mesentery to the lung. We demonstrated that LPT enhanced the clearance of S. pneumoniae after 3 consecutive daily treatments and found that LPT increased the concentrations of SP-D, IL-6, IL-12p70, and IL-17 in BALF and enhanced the release of NO2- and IL-6 by AM 4 days post-infection. Collectively these studies suggest, that LPT re-distributes inflammatory mediators to the lung, enhances the recruitment of macrophages and neutrophils to the lung and skews alveolar macrophages towards a M1 phenotype, all of which may be responsible for and promote the clearance of S. pneumoniae.Item Lymphatic Pump Treatment Mobilizes Leukocytes from the Gastrointestinal Associated Lymphoid Tissue(2008-12-01) Bearden, Melissa K.; Simecka, Jerry; Downey, H. Fred; Machu, TinaBearden, Melissa K., Lymphatic Pump Treatment Mobilizes Leukocytes from the Gastrointestinal Associated Lymphoid Tissue. Master of Science (Microbiology and Immunology), December, 2008, 39 pp., 4 tables, 5 illustrations, references, 40 titles. Lymphatic flow and the migration ofleukocytes are important to maintaining health. One treatment used to improve lymph flow is abdominal lymphatic pump treatment (LPT), which is thought to increase lymph return from the abdominal area. Previous research has shown the LPT increases lymph flow and leukocyte numbers in thoracic duct lymph, but further investigation into the specific cell types and their sources are needed. Through sampling the thoracic and intestinal lymph ducts, as well as the mesenteric lymph nodes (MLN), it was seen that there is an increase in cell output in the thoracic and intestinal ducts and the MLN during LPT as compared to baseline. This increase in cell output may help to augment the immune response during infection.Item The Effects of Osteopathic Manipulation Techniques on the Lymphatic System(2021-05) Parker, Madison A.; Hodge, Lisa M.; Tune, Johnathan; Mallet, Robert T.Osteopathic manipulation techniques (OMT) have been utilized by osteopathic physicians to treat a variety of conditions including musculoskeletal dysfunctions, edema, inflammation, and disease. However, the mechanisms by which OMT aides the body in healing are not well understood. The long-term goal of our research is to advance our understanding of the impact of OMT during inflammatory disease, such as sepsis. The purpose of this study was to develop a swine model and to establish surgical techniques that will be used in future studies investigating the impact of OMT on the lymphatic system during disease. We hypothesized that the abdominal lymphatic pump technique (LPT) would enhance thoracic duct lymph (TDL) flow. Four swine subjects (two male and two female) were placed under anesthesia. The thoracic duct was exposed via thoracotomy then cannulated using an angio-catheter. TDL was collected from the four pigs during four-minutes of baseline, four-minutes of LPT, and four minutes post-LPT. TDL flow was measured by timed collection during each condition. TDL was centrifuged to remove the cellular components, and the supernatant was stored for biomarker analysis. Results: TDL flow at baseline was 2.2 ± 1.0 mL/min and LPT increased lymph flow rate to 5.58 ± 1.8 mL/min. In two experiments, thoracic lymph nodes and thoracic duct lymph were collected, and leukocyte were isolated to optimize the flow cytometry staining protocol. In our pilot study, LPT increased TDL flow approximately 2-fold in our swine subjects and demonstrated surgical feasibility. In future studies we will study the physiological effects of OMT, including LPT, during sepsis. This knowledge would provide an evidence-based foundation for the use, or contraindication, of OMT during sepsis and aid osteopathic physicians during their therapeutic decision making.