Evaluation of the AmpFlSTR® MiniFiler Typing Kit: Mixture Studies and Non-Probative Sample Studies

This project was aimed to assemble and assess data from internal validations of AmpFlSTR® MiniFilerTM PCR Amplification Kit by forensic laboratories across the United States. After compilation, data was evaluated for quality of testing, results, and concordance within and between participating laboratories. It was concluded that MiniFilerTM can successfully amplify DNA from multiple sources in mixtures of neat and degraded samples, as well as enhance DNA profiles obtained for several types of samples with suspected PCR inhibition or degradation. The data was collected into a final report with discussions and conclusions to the findings for submission to the National DNA Indexing System (NDIS) Approval Board for authorization to use forensic DNA genotypes generated using MiniFilerTM in national DNA databases.