Typing Success of Short Tandem Repeat Loci on Skeletal Remains: Comparison of the AmpFLSTR® Identifiler® Plus and GlobalFiler™ PCR Amplification Kit.

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dc.contributor.advisorArthur Eisenberg
dc.creatorGarcia, Stephanie A.
dc.date.accessioned2019-08-22T20:46:19Z
dc.date.available2019-08-22T20:46:19Z
dc.date.issued2014-05-01
dc.date.submitted2014-05-22T08:00:05-07:00
dc.description.abstractThe AmpFLSTR® Identifiler® Plus PCR Amplification Kit (Life Technologies, Carlsbad, CA), and the recently released GlobalFiler™ PCR Amplification Kit (Life Technologies) were compared to determine which provided the greater power of discrimination from DNA extracted from skeletal remains. I hypothesized, STR profiles obtained using the GlobalFiler™ kit for analysis of skeletal remains would result in an increased number of reportable genetic loci, and provide greater power of discrimination as compared to the Identifiler® Plus Kit. The results of this study showed GlobalFiler™, along with an automated extraction, could produce comparable or greater genetic typing results and discriminatory power from skeletal remains without the use of a second amplification kit.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28757
dc.language.isoen
dc.provenance.legacyDownloads521
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectMedical Genetics
dc.subjectTyping
dc.subjectIdentifiler Plus
dc.subjectGlobalFiler
dc.subjectShort Tandem Repeats
dc.subjectSkeletal remains
dc.subjectDNA
dc.subjectForensic Genetics
dc.subjectKits
dc.titleTyping Success of Short Tandem Repeat Loci on Skeletal Remains: Comparison of the AmpFLSTR® Identifiler® Plus and GlobalFiler™ PCR Amplification Kit.
dc.typeThesis
dc.type.materialtext
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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