A Synthetic Formula Amino Acid Diet Leads to Microbiome Dysbiosis, Reduced Colon Length, Inflammation, and Altered Locomotor Activity in C57BL/6J Mice

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dc.creatorMancilla, Viviana J.
dc.creatorBraden-Kuhle, Paige N.
dc.creatorBrice, Kelly N.
dc.creatorMann, Allison E.
dc.creatorWilliams, Megan T.
dc.creatorZhang, Yan
dc.creatorChumley, Michael J.
dc.creatorBarber, Robert C.
dc.creatorWhite, Sabrina N.
dc.creatorBoehm, Gary W.
dc.creatorAllen, Michael S.
dc.creator.orcid0000-0003-2293-8078 (Allen, Michael S.)
dc.creator.orcid0000-0001-6857-0286 (Barber, Robert C.)
dc.creator.orcid0000-0001-5784-4659 (Zhang, Yan)
dc.date.accessioned2023-12-01T20:27:50Z
dc.date.available2023-12-01T20:27:50Z
dc.date.issued2023-11-25
dc.description.abstractThe effects of synthetic, free-amino acid diets, similar to those prescribed as supplements for (phenylketonuria) PKU patients, on gut microbiota and overall health are not well understood. In the current, multidisciplinary study, we examined the effects of a synthetically-derived, low-fiber, amino acid diet on behavior, cognition, gut microbiome composition, and inflammatory markers. A cohort of 20 male C57BL/6J mice were randomly assigned to either a standard or synthetic diet (n = 10) at post-natal day 21 and maintained for 13 weeks. Sequencing of the 16S rRNA gene from fecal samples revealed decreased bacterial diversity, increased abundance of bacteria associated with disease, such as Prevotella, and a downward shift in gut microbiota associated with fermentation pathways in the synthetic diet group. Furthermore, there were decreased levels of short chain fatty acids and shortening of the colon in mice consuming the synthetic diet. Finally, we measured TNF-alpha, IL-6, and IL-10 in serum, the hippocampus, and colon, and found that the synthetic diet significantly increased IL-6 production in the hippocampus. These results demonstrate the importance of a multidisciplinary approach to future diet and microbiome studies, as diet not only impacts the gut microbiome composition but potentially systemic health as well.
dc.description.sponsorshipThis research was funded in part by the National Institute on Minority Health and Health Disparities of the National Institutes of Health under Award Number S21MD012472 and by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R25GM125587. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
dc.identifier.citationMancilla, V. J., Braden-Kuhle, P. N., Brice, K. N., Mann, A. E., Williams, M. T., Zhang, Y., Chumley, M. J., Barber, R. C., White, S. N., Boehm, G. W., & Allen, M. S. (2023). A Synthetic Formula Amino Acid Diet Leads to Microbiome Dysbiosis, Reduced Colon Length, Inflammation, and Altered Locomotor Activity in C57BL/6J Mice. Microorganisms, 11(11), 2694. https://doi.org/10.3390/microorganisms11112694
dc.identifier.issn2076-2607
dc.identifier.issue11
dc.identifier.urihttps://hdl.handle.net/20.500.12503/32442
dc.identifier.volume11
dc.publisherMDPI
dc.relation.urihttps://doi.org/10.3390/microorganisms11112694
dc.rights.holder© 2023 by the authors.
dc.rights.licenseAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceMicroorganisms
dc.subjectSCFA
dc.subjectbutyrate
dc.subjectfiber
dc.subjectgut-brain axis
dc.subjectinflammation
dc.subjectmouse model
dc.subjectprebiotics
dc.subjectsynthetic diet
dc.titleA Synthetic Formula Amino Acid Diet Leads to Microbiome Dysbiosis, Reduced Colon Length, Inflammation, and Altered Locomotor Activity in C57BL/6J Mice
dc.typeArticle
dc.type.materialtext

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