Creating novel purification and biochemical characterization protocols for C. collagenase from Clostridium histolyticum, developing a new emergency medicine product, and formulating several novel therapies for chronic and acute wound treatment

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dc.contributor.advisorJerry W. Simecka
dc.contributor.committeeMemberPatricia A. Gwirtz
dc.contributor.committeeMemberAleksa Jovanovic
dc.creatorMars, Jason P.
dc.date.accessioned2019-08-22T21:18:54Z
dc.date.available2019-08-22T21:18:54Z
dc.date.issued2017-05-01
dc.date.submitted2017-05-12T08:54:07-07:00
dc.description.abstractThe pharmaceutical industry not only includes infinite areas of specialization, but also consists of distinct areas that do not typically overlap. Biotechnology is the branch of medicinal research that bridges the gap between the fields within the pharmaceutical industry by being able to take on the challenges that require knowledge of a vast range of information. This practicum was organized to put the scientific knowledge and the interdisciplinary practices of biotechnology to use in a modern day, pharmaceutical company specializing in wound therapy and skincare: Smith & Nephew Biotherapeutics. Wound therapy has the widest range of application due to being one of the few fields that affects everyone, regardless of medical disposition. The specific goals of this practicum were: to develop novel purification and biochemical characterization protocols for C. collagenase from Clostridium histolyticum to replace current production methods of Santyl®, to develop a working prototype of a venom-based, hemostatic film, and perform reformulation, quality control, troubleshooting, and verification testing on samples of Regranex®, Iodosorb “Max”, and EU-Collagenase. Every goal presented was approached with the end results of saving Smith & Nephew costs, reducing bioburden of production, and creating more efficient protocols to bring Smith & Nephew into the modern age.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29238
dc.language.isoen
dc.provenance.legacyDownloads98
dc.subjectBacteria
dc.subjectEnzymes and Coenzymes
dc.subjectMedical Biotechnology
dc.subjectTherapeutics
dc.subjectbiotechnology
dc.subjectcollagenase
dc.subjectClostridium histolyticum
dc.subjecthemostatic bandage
dc.subjectwounds
dc.titleCreating novel purification and biochemical characterization protocols for C. collagenase from Clostridium histolyticum, developing a new emergency medicine product, and formulating several novel therapies for chronic and acute wound treatment
dc.typeInternship Practicum Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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