The Role of Loop C of the 5-Hydroxytryptamine3A Receptor in Ligand Recognition

Simple item page
dc.contributor.advisorMachu, Tina K.
dc.contributor.committeeMemberDillon, Glenn
dc.contributor.committeeMemberSingh, Meharvan
dc.creatorLote, Rashmi R.
dc.date.accessioned2019-08-22T20:36:48Z
dc.date.available2019-08-22T20:36:48Z
dc.date.issued2006-01-01
dc.date.submitted2014-02-24T14:54:05-08:00
dc.description.abstractThe 5-Hydroxytryptamine3 (the 5-HT3) receptor is composed of homomers of A subunits or heteromers of A and B subunits. The discovery of the crystal structure of the acetylcholine binding protein (Brejc et al., 2001) has helped us identify the generalized structure of the N-terminal domains of this superfamily, but the precise details regarding the amino acid residues involved in the process of ligand binding in the 5-Ht3 receptor are unknown. Mouse and human 5-HT3A receptors are 84% identical at the amino acid level, yet they have differential sensitivities to numerous drugs that bind to the ligand recognition site, for example, agonists such as 2-Methyl serotonin and m-Chlorophenyl Biguanide (m-CPBG) and competitive antagonists such as d-Tubocurarine (curare). The distal 1/3 part of the N-terminal domain, which contains both Loop C and Loop F is responsible for determining the potency of curare of the 5-HT3A receptor, these loops consist of thirteen non-conserved amino acid residues between mouse and human the 5-HT3A receptors. As a result of these differences, curare is 135-fold more potent at the mouse wild-type receptor than human wild-type receptor. In this project we utilized the differential curare sensitivity of mouse and human 5-HT3A receptors to obtain information regarding the amino acid residues in Loop C, involved in ligand binding process. Chimeric and point mutant receptors were constructed on the human receptor background with substitutions of corresponding mouse orthologs and expressed in Xenopus oocytes. Curare potency was assessed with two-electrode voltage clamp electrophysiological recordings. Our results suggests that a minimum of four mouse orthologs are required to produce the curare IC50 of 41 nM obtained in the chimeric receptor, which contains seven mouse orthologs (Hch Loop C receptor).
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/28580
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectDiseases
dc.subjectLife Sciences
dc.subjectMedical Neurobiology
dc.subjectMedicine and Health Sciences
dc.subjectMolecular and Cellular Neuroscience
dc.subjectNervous System
dc.subjectNeuroscience and Neurobiology
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Neuroscience and Neurobiology
dc.subject5-Hydroxytryptamine3
dc.subject5HT3
dc.subjecthomomers
dc.subjectheteromers
dc.subjectA subunit
dc.subjectB subunit
dc.subjectacetylcholine binding protein
dc.subject5-HT3A
dc.subjectcurare sensitivity
dc.subjectmice
dc.subjectligand binding process
dc.subjectmouse orthologs
dc.subjectxenopus oocytes
dc.subjectelectrophysiological recordings
dc.subjectchimeric receptor
dc.titleThe Role of Loop C of the 5-Hydroxytryptamine3A Receptor in Ligand Recognition
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Lote_TheRoleOfLoop.pdf
Size:
25.86 MB
Format:
Adobe Portable Document Format
Download

Collections

School of Biomedical Sciences
Theses and Dissertations