The Role of 14-3-3 in the Signaling of Cardiac Hypertrophy

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dc.contributor.advisorStephen R. Grant
dc.contributor.committeeMemberNeeraj Agarwall
dc.contributor.committeeMemberGlenn Dillon
dc.creatorEllis, Joel James
dc.date.accessioned2019-08-22T21:24:47Z
dc.date.available2019-08-22T21:24:47Z
dc.date.issued2002-01-01
dc.date.submitted2014-05-05T12:18:05-07:00
dc.description.abstractEllis, Joel J., The Role of 14-3-3 in the Signaling of Cardiac Hypertrophy. Master of Science (Biotechnology), January, 2002, 97pp., 21 illustrations, bibliography, 46 titles. The METF2 family of transcription factors is regulated by class II histone deacetylaces in the nucleus. MEF2-dependent gene expression in cardiomyocytes is augmented by the 14-3-3 chaperone family which binds and sequesters class II HDACs in the cytoplasm upon the activation of CaM kinase I & IV. A 14-3-3β mutant was made by conservatively substituting aspartate for serine 60 and serine 65. In MEF2 enhancer-reporter transfection assays, expression of the 14-3-3β double mutant silenced transcription mediated by CaM KI & IV in both cardiomyocytes and vascular smooth muscle cells. Co-expression of the 14-3-3β double mutant was also able to suppress MEF2 enhancer activation by phenylephrine in cardiomyocytes and vascular smooth muscle cells. Mammalian two-hybrid cloning of the 14-3-3β wild-type and double mutant genes will allow analysis of the protein-protein interaction between the different 14-3-3β monomers. These data suggest that 14-3-3β plays a critical role in the silencing of MEF2 mediated hypertrophy-sensitive gene transcription.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29313
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell Anatomy
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectRole
dc.subject14-3-3
dc.subjectcardiac hypertrophy
dc.subjectsignaling
dc.subjecttranscription factors
dc.subjectMETF2
dc.subjectclass II histone deacetylaces
dc.subjectnucleus
dc.subjectcardiomyocytes
dc.subject14-3-3β
dc.subjectmutant genes
dc.subjectvascular smooth muscle cells
dc.subjectcloning
dc.subjecthypertrophy-sensitive gene transcription
dc.titleThe Role of 14-3-3 in the Signaling of Cardiac Hypertrophy
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiotechnology
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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