Analysis of mitochondrial protein sulfenation during aging in the rat brain
dc.creator | Yan, Liang-Jun | |
dc.date.accessioned | 2019-08-22T19:52:40Z | |
dc.date.available | 2019-08-22T19:52:40Z | |
dc.date.issued | 2019-03-05 | |
dc.date.submitted | 2019-01-07T13:20:21-08:00 | |
dc.description.abstract | Purpose: The purpose of the present study was to identify mitochondrial proteins that undergo changes in cysteine sulfenation during aging. Methods: Studies were conducted in rats when they were 5 or 30 months of age. Following blocking of free protein thiols with N-ethylmaleimide, protein sulfenic acids were reduced by arsenite to free thiol groups that were subsequently labeled with biotin-maleimide. Samples were then comparatively analyzed by 2-dimensional Western blots, and proteins showing changes in sulfenation were selectively identified by mass spectrometry peptide sequencing. Result: Five proteins were identified. Proteins showing an age-related decrease in sulfenation include pyruvate carboxylase and pyruvate dehydrogenase; while those showing an age-related increase in sulfenation include aconitase, mitofilin, and tubulin (α-1). Conclusion: Results of the present study provide a general picture of mitochondrial protein sulfenation in brain oxidative stress and implicate the involvement of protein sulfenation in overall decline of mitochondrial function during brain aging. | |
dc.identifier.uri | https://hdl.handle.net/20.500.12503/27155 | |
dc.language.iso | en | |
dc.provenance.legacyDownloads | 0 | |
dc.title | Analysis of mitochondrial protein sulfenation during aging in the rat brain | |
dc.type | poster | |
dc.type.material | text |