Discovery and Characterization of Tetranucleotide Short Tandem Repeats in North American Bears (Ursids)

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dc.contributor.advisorJohn V. Planz
dc.creatorGraham, Michelle L.
dc.date.accessioned2019-08-22T21:02:04Z
dc.date.available2019-08-22T21:02:04Z
dc.date.issued2015-05-01
dc.date.submitted2015-07-08T08:36:33-07:00
dc.description.abstractAccurate individual identification is essential to wildlife crime investigations and conservation genetics. Current methodology utilizes dinucleotide short tandem repeats (STRs) that can be difficult to type accurately and have high mutation rates; however, tetranucleotide STRs have greater stability and allele diversity. The main objective of this study was to identify potential tetranucleotide STR loci and internal variants for the American black bear, brown bear, and polar bear. Barcoded genome libraries were prepared for each species from extracted and enzymatically fragmented DNA, size selected, quantified, enriched using biotinylated RNA baits to capture twelve common mammalian sequence motifs, and massively parallel sequenced. One potential locus was identified using the NextGENe® software and six potential loci were identified using algorithm mining.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29015
dc.language.isoen
dc.provenance.legacyDownloads200
dc.subjectAnimals
dc.subjectForensic Science and Technology
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectMolecular Biology
dc.subjectMolecular Genetics
dc.subjectUrsids
dc.subjectshort tandem repeats
dc.titleDiscovery and Characterization of Tetranucleotide Short Tandem Repeats in North American Bears (Ursids)
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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