Evaluation of the AluQuant Human DNA Quantitation System Using the 96-Well Plate Format

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dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.creatorAlexander, Uvonna Faye Lewallen
dc.date.accessioned2019-08-22T21:33:32Z
dc.date.available2019-08-22T21:33:32Z
dc.date.issued2002-08-01
dc.date.submitted2014-05-01T06:48:05-07:00
dc.description.abstractAlexander, Uvonna Faye Lewallen, Evaluation of the AluQuant Human DNA Quantitation System Using the 96-Well Plate Format. Master of Science (Forensic Genetics), August 2002, 121 pp., 4 charts, 4 tables, 8 figures, 2 appendices, references, 13 titles. This study evaluated the AluQuant Human DNA Quantitation System (Promega Corporation, Madison, WI) using the 96-well plate format for possible implementation by Orchid Cellmark Dallas. The importance of human DNA quantitation in forensics is two-fold. First, the Quality Assurance Standards set forth by the DNA Advisory Board requires human DNA in forensic samples be quantitated. Also, the highly sensitive PCR multiplex PCR multiplex assays used in forensics have been optimized for a narrow range of template DNA, thus requiring accurate and consistent quantitation. This evaluation consisted of three general goals: examination of the Reporter Microplate Luminometer (Turner BioSystems, Sunnyvale, CA), alteration of the assay variables to obtain optimal performance, and characterization of the assay. The Reporter produces reproducible results and is sensitive to at least 4.88 x 10^-9 moles ATP. Of the variables tested, quick centrifugation of the incubation plate had the most noticeable effect on the results obtained. The assay did not perform as characterized by Promega. AluQuant is not reproducible, nor does it consistently produce results within a two-fold accuracy range. Therefore, Orchid Cellmark Dallas will not be implementing the AluQuant Assay.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29422
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectBiomedical Devices and Instrumentation
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectCells
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectLife Sciences
dc.subjectMedical Cell Biology
dc.subjectMedical Genetics
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectAluQuant Human DNA Quantification System
dc.subject96-Well Plate Format
dc.subjectPromega
dc.subject96 well plate format
dc.subjectOrchid Cellmark Dallas
dc.subjectPCR multiplex
dc.subjectquantitation
dc.subjectReporter Microplate Luminometer
dc.subjectassay variables
dc.subjectoptimal performance
dc.subjectassay characterization
dc.subjectcentrifugation
dc.subjectincubation plate
dc.titleEvaluation of the AluQuant Human DNA Quantitation System Using the 96-Well Plate Format
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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