Next-generation sequencing and cytokine analysis of bronchoalveolar lavage samples from mechanically ventilated trauma patients
| dc.creator | Smith, Ashley D. | |
| dc.creator | Zhang, Yan | |
| dc.creator | Huebinger, Ryan M. | |
| dc.creator | Ireland, Sara J. | |
| dc.creator | Monson, Nancy L. | |
| dc.creator | Allen, Michael | |
| dc.date.accessioned | 2019-08-22T19:39:08Z | |
| dc.date.available | 2019-08-22T19:39:08Z | |
| dc.date.issued | 2015-03 | |
| dc.date.submitted | 2015-03-05T10:14:55-08:00 | |
| dc.description.abstract | Purpose: Mechanically ventilated trauma patients are at high risk for ventilator-associated pneumonia (VAP). VAP diagnosis relies on several clinical factors including the identification of a specific pathogen by culture-dependent techniques. Often, patients exhibit signs of VAP, yet the hospital lab is unable to culture a pathogen from a bronchoalveolar lavage (BAL) sample. We hypothesize that these culture-negative, presumptive positive patients are infected with potentially pathogenic bacteria that are not detected by traditional culture techniques. Methods: Culture-positive and -negative hospital results were tested again by Sanger and next-generation sequencing (NGS) on the Ion Torrent PGM. Sample cytokine levels were determined using a Bio-Plex Pro Human Th17 cytokine panel. Results: No significant difference was seen between the identification methods in culture-positive BAL. However, NGS analysis of culture-negative BAL identified hundreds of bacterial genera, including a group of patients that exhibited similar bacterial composition, diversity, and abundance. This group was significantly different from other culture-negative BAL that were dominated by one or two suspected pathogens. Culture-negative BAL contained significantly less IL-1β, IL-6 and TNF-α than culture-positive BAL. Conclusions: NGS is a valuable method for pathogen identification, particularly for difficult to culture BAL. Culture-positive BAL exhibit less bacterial diversity and increased cytokine production than culture-negative BAL. The grouping of culture-negative BAL with similar characteristics may denote a core lung microbiome. | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12503/26583 | |
| dc.language.iso | en | |
| dc.title | Next-generation sequencing and cytokine analysis of bronchoalveolar lavage samples from mechanically ventilated trauma patients | |
| dc.type | oral | |
| dc.type.material | text |