Novel androgen receptor splice variant in the substantia nigra

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dc.contributor.advisorCunningham, Rebecca L.
dc.contributor.committeeMemberBasha, Riyaz
dc.contributor.committeeMemberSalvatore, Michael
dc.creatorContreras, Jo Garza
dc.date.accessioned2019-08-22T21:39:58Z
dc.date.available2019-08-22T21:39:58Z
dc.date.issued2017-08-01
dc.date.submitted2017-10-13T13:06:14-07:00
dc.description.abstractTestosterone can increase calcium influx and cell death in dopamine neurons via a putative membrane androgen receptor (mAR). The mAR induced calcium increase may be due to activation of G-proteins involved in calcium mobilization. Previous studies using an N-terminal targeted androgen receptor (AR) antibody yielded low AR expression in dopamine neurons. Studies in our lab show high AR expression using a C-terminal targeted AR antibody. This difference in expression may be due to an AR variant. We hypothesize an AR variant is present in the membrane of dopaminergic neurons and associated with G proteins. To identify the presence of AR in dopaminergic neurons. We performed immunoblot, sucrose gradient, and immunohistochemistry studies. To determine the protein-protein interaction between mAR and G-proteins we performed co-immunoprecipitation studies. Our results show AR45 localizes in the membrane lipid rafts of dopaminergic neurons. Furthermore, AR45 interacts with Gαq and Gαo G-proteins, which can impact calcium signaling.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29497
dc.language.isoen
dc.provenance.legacyDownloads22
dc.subjectAmino Acids, Peptides, and Proteins
dc.subjectHormones, Hormone Substitutes, and Hormone Antagonists
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectNervous System Diseases
dc.subjectaging
dc.subjecttestosterone
dc.subjectandrgogen receptor variant
dc.subjectAR45
dc.subjectParkinson Disease
dc.titleNovel androgen receptor splice variant in the substantia nigra
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineBiomedical Sciences
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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