Validation of Genemapper ID Human Identification Software for Forensic STR DNA Analysis

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dc.contributor.advisorJohn Planz
dc.contributor.committeeMemberArthur Eisenberg
dc.contributor.committeeMemberJoseph Warren
dc.creatorCapt, Christina L.
dc.date.accessioned2019-08-22T21:22:37Z
dc.date.available2019-08-22T21:22:37Z
dc.date.issued2005-12-01
dc.date.submitted2014-05-02T09:00:05-07:00
dc.description.abstractCapt, Christina L., Validation of GeneMapper ID Human Identification Software for Forensic STR DNA Analysis. Master of Science (Forensic Genetics), December, 2005, 73 pp., 5 tables, 12 figures, references, 6 titles. ABI GeneMapper ID analysis software replaces and combines both GeneScan and Genotyper data analysis programs. Fragment sizing and allele typing functions are performed in a single analysis, and the software includes data quality assessment features not available with previous software packages. The software was directly compared to the current laboratory STR data analysis software, GeneScan and Genotyper. All peaks evaluated with GeneMapper ID exhibited lower peak heights that their GeneScan/Genotyper analysis counterparts. A mean percent decrease in peak height of 3.8% ± 0.9% was observed for all peaks greater than 500rfu. Observed stutter ratios were comparable to the default stutter filter settings of GeneMapper ID. Parallel analyses of 388 sample files resulted in absolute concordance for all reference samples and most evidentiary samples. The software performed better than GeneScan/Genotyper in labeling microvariants, baselining data, disregarding –A peaks, aligning below threshold data, and defining size standard peaks despite artifact interference. The Off Scale and Spectral Pull-up PQV did not function as expected for sizing and genotyping STR fragments for forensic human identification analysis.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29285
dc.language.isoen
dc.provenance.legacyDownloads0
dc.subjectCell and Developmental Biology
dc.subjectComputational Biology
dc.subjectEquipment and Supplies
dc.subjectForensic Science and Technology
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectGenetic Structures
dc.subjectHealth Information Technology
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Genetics and Genomics
dc.subjectGeneMapper ID Human Identification Software
dc.subjectForensic STR DNA Analysis
dc.subjectGeneScan
dc.subjectGenotyper
dc.subjectDNA analysis
dc.subjectsoftware performance
dc.subjectgenotyping STR fragments
dc.subjectforensic science
dc.subjecthuman identification analysis
dc.titleValidation of Genemapper ID Human Identification Software for Forensic STR DNA Analysis
dc.typeProfessional Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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