Alternative procedure for DNA recovery from cartridges and casings

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dc.contributor.advisorPlanz, John V.
dc.contributor.committeeMemberJones, Harlan P.
dc.contributor.committeeMemberWarren, Joseph E.
dc.creatorBettex, Kelsey D.
dc.date.accessioned2020-04-22T19:28:52Z
dc.date.available2020-04-22T19:28:52Z
dc.date.issued2020-05
dc.description.abstractBoth unfired and fired ammunition recovered from a crime scene are useful in connecting a crime to a specific firearm, however its use in identifying individuals is less common due to low-level or degraded DNA present. Traditionally, the standard procedure for DNA sampling of ammunition evidence is the use of a swabbing technique. The New York State Police (NYSP) Forensic Investigation Center conducted a study to test the efficacy of an alternative method for obtaining DNA from cartridges and casings, soaking the sample prior to extraction. This method presented a 186% increase in total DNA yield, 169% increase in male DNA yield, and a 44% increase in comparable profiles obtained using GlobalFiler(TM) and 240% using Yfiler(R) Plus. Results showed fewer 'no data' determinations using the soaking method, indicating more DNA obtained. Although a statistically significant difference was not observed between the two methods (p = 0.070), a qualitative significance was.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29837
dc.language.isoen
dc.subjectammunition
dc.subjectcartridges
dc.subjectcasings
dc.subjectsoaking method
dc.subjectswabbing method
dc.subjecttrace DNA
dc.subject.meshForensic Genetics / methods
dc.subject.meshForensic Sciences / methods
dc.subject.meshFirearms
dc.subject.meshDNA / isolation & purification
dc.subject.meshSpecimen Handling / methods
dc.subject.meshNew York
dc.titleAlternative procedure for DNA recovery from cartridges and casings
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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