Optimization of Spermatozoa Capture During the Differential Extraction Process for STR Typing with the Potential for Automation

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dc.contributor.advisorEisenberg, Arthur
dc.contributor.committeeMemberMartin, Michael W.
dc.contributor.committeeMemberWordinger, Robert J.
dc.creatorMarshall, Pamela L.
dc.date.accessioned2019-08-22T21:04:49Z
dc.date.available2019-08-22T21:04:49Z
dc.date.issued2002-05-01
dc.date.submitted2013-09-13T08:54:07-07:00
dc.description.abstractMarshall, Pamela. Optimization of Spermatozoa Capture During the Differential Extraction Process for STR Typing With the Potential for Automation. Master of Science (Forensic Genetics). May, 2002. In 1998, within the United States, it is estimated that a rape occurred every 2.3 minutes. In 1995, according to the Bureau of Justice Statistics, an estimated 350,000 rapes or sexual assaults (R/SA0 were experienced by persons age 12 or older. Of the estimated 100,000 R/SA reported, there were only approximately, 25,000 cases analyzed by crime labs nationwide. The majority of crime laboratories throughout the U.S., especially those in major metropolitan cities, have a significant backlog of unresolved R/SA cases. With the implementation of the Convicted Offender Database (CODIS), it is essential that all R/SA cases by analyzed, especially those lacking a known suspect. The comparison of the short tandem repeat (STR) profiles derived from sperm DNA recovered from evidentiary material with CODIS samples would provide the police with critical investigative leads resulting in the identification of the assailant. The goal of this research is to develop a cellular sorting method for the isolation of sperm cells from sexual assault samples which will: 1) take advantage of differentiating features (extracellular antigenic sites) for complete separation of cell types, 2) provide a more efficient means of sperm recovery, increasing DNA yield from the male fraction, and 3) ensure the DNA isolation process is compatible with the amplification of the CODIS core STR loci. Overall, the proposed technique will increase the probability of success in the analysis of sexual assault case samples. (NIJGrant #: 2000-IJ-CX-K009).
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29052
dc.language.isoen
dc.provenance.legacyDownloads2
dc.subjectBiology
dc.subjectBiomedical Engineering and Bioengineering
dc.subjectBiotechnology
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.subjectEquipment and Supplies
dc.subjectFluids and Secretions
dc.subjectGenetics
dc.subjectGenetics and Genomics
dc.subjectInvestigative Techniques
dc.subjectLife Sciences
dc.subjectMedical Genetics
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectOther Cell and Developmental Biology
dc.subjectOther Genetics and Genomics
dc.subjectOptimization
dc.subjectspermatozoa capture
dc.subjectdifferential extraction process
dc.subjectshort tandem repeat
dc.subjectautomation
dc.subjectCODIS
dc.subjectsexual assault
dc.subjectDNA
dc.subjectgenetics
dc.titleOptimization of Spermatozoa Capture During the Differential Extraction Process for STR Typing with the Potential for Automation
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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