Developmental and Internal Validation of a Mitochondrial DNA Direct Amplification Kit for Forensic Reference Samples

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dc.contributor.advisorArthur J. Eisenberg
dc.contributor.committeeMemberRhonda Roby
dc.contributor.committeeMemberDixie L. Peters
dc.creatorAlicea-Centeno, Alessandra
dc.date.accessioned2019-08-22T21:50:58Z
dc.date.available2019-08-22T21:50:58Z
dc.date.issued2015-05-01
dc.date.submitted2015-07-08T08:36:33-07:00
dc.description.abstractEvaluation of the control region of the mitochondrial genome is a common practice for forensic casework and research purposes. Since no kit is currently commercially available for the amplification of mitochondrial DNA (mtDNA), its sequencing procedure is time-consuming and laborious. Six steps are generally followed: DNA extraction, quantification and normalization, amplification of two regions (hypervariable regions 1 and 2), cycle sequencing, capillary electrophoresis and data analysis. This project evaluated a mtDNA direct amplification kit by performing developmental and internal validations. The studies performed included sensitivity, stability, reproducibility, case- type samples, mixtures and accuracy. The mtDNA direct amplification kit successfully amplified reference samples used in each study without the need of extraction and quantification steps. In addition, mtDNA profiles were obtained from the sequenced amplification products. Using the validated direct amplification procedure in the laboratory will improve workflow, decrease operational cost and reduce the possibility of error by minimizing sample handling.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/29627
dc.language.isoen
dc.provenance.legacyDownloads118
dc.subjectForensic Science and Technology
dc.subjectGenetics and Genomics
dc.subjectInvestigative Techniques
dc.subjectLaboratory and Basic Science Research
dc.subjectMedical Sciences
dc.subjectMedicine and Health Sciences
dc.subjectmitochondrial DNA
dc.subjectvalidation
dc.subjectdirect amplification
dc.titleDevelopmental and Internal Validation of a Mitochondrial DNA Direct Amplification Kit for Forensic Reference Samples
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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