Internal Validation of the AmpFlSTR® Yfiler™ PCR Amplification Kit

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dc.contributor.advisorEisenberg, Arthur
dc.creatorAbraham, Lena
dc.date.accessioned2019-08-22T19:55:58Z
dc.date.available2019-08-22T19:55:58Z
dc.date.issued2009-08-01
dc.date.submitted2010-03-17T12:31:56-07:00
dc.description.abstractThe project conducted at the Forensic Testing Laboratories (FTL) in Las Cruces, NM involved an internal validation of the AmpFlSTR® Yfiler™ PCR Amplification kit. FTL currently implements the PowerPlex® Y kit which amplifies eleven Y-STR markers. The Yfiler™ kit provides more discriminatory power as it contains sixteen Y-STR markers. The validation included sensitivity, mixture, case type samples, contamination, precision and injection time change studies as required by the National Standards. Based on the data obtained, the Yfiler™ kit successfully detects male DNA in the presence of female DNA, and the correct number of male contributors in male/male mixtures is distinguishable. The additional loci included in the Yfiler™ kit can provide more insight than what is achieved through the PowerPlex® Y kit.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/20.500.12503/27325
dc.language.isoen
dc.provenance.legacyDownloads60
dc.subjectinternal validation
dc.subjectyfiler kit
dc.titleInternal Validation of the AmpFlSTR® Yfiler™ PCR Amplification Kit
dc.typeInternship Practicum Report
dc.type.materialtext
thesis.degree.departmentGraduate School of Biomedical Sciences
thesis.degree.disciplineForensic Genetics
thesis.degree.grantorUniversity of North Texas Health Science Center at Fort Worth
thesis.degree.nameMaster of Science

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