Characterization of Tear Immunoglobulins in a Small-Cohort of Keratoconus Patients

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dc.creatorMcKay, Tina B.
dc.creatorSerjersen, Henrik
dc.creatorHjortdal, Jesper
dc.creatorZieske, James D.
dc.creatorKaramichos, Dimitrios
dc.creator.orcid0000-0002-8761-3824 (Karamichos, Dimitrios)
dc.date.accessioned2022-09-20T20:19:47Z
dc.date.available2022-09-20T20:19:47Z
dc.date.issued2020-06-10
dc.description.abstractKeratoconus (KC) is classically considered a non-inflammatory condition caused by central corneal thinning that leads to astigmatism and reduced visual acuity. Previous studies have identified increased systemic levels of pro-inflammatory factors, including interleukin-6, tumor necrosis factor-alpha, and matrix metalloproteinase-9, suggesting that KC may have an inflammatory component in at least a subset of patients. In this study, we evaluated the levels of different immunoglobulins (light and heavy chains) based on Ig alpha, Ig lambda, Ig kappa, Ig micro, and Ig heavy chain subunits in non-KC tears (n = 7 control individuals) and KC tears (n = 7 KC patients) using tandem-liquid chromatography mass spectrometry. The most abundant Ig heavy chains detected in both control individuals and KC patients were Ig alpha-1 and Ig alpha-2 likely correlating to the higher IgA levels reported in human tears. We identified significant differences in immunoglobulin kappa-chain V-II levels in KC patients compared to control individuals with no significant difference in Ig kappa/Ig lambda ratios or heavy chain levels. Our study supports previous findings suggesting that KC possesses a systemic component that may contribute to the KC pathology. Further studies are required to define causality and establish a role for systemic immune system-dependent factors and pro-inflammatory processes in KC development or progression.
dc.description.sponsorshipThis research was funded by the National Institutes of Health and the National Eye Institute, EY028888.
dc.identifier.citationMcKay, T. B., Serjersen, H., Hjortdal, J., Zieske, J. D., & Karamichos, D. (2020). Characterization of Tear Immunoglobulins in a Small-Cohort of Keratoconus Patients. Scientific reports, 10(1), 9426. https://doi.org/10.1038/s41598-020-66442-7
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/20.500.12503/31789
dc.identifier.volume10
dc.publisherSpringer Nature
dc.relation.urihttps://doi.org/10.1038/s41598-020-66442-7
dc.rights.holder© The Author(s) 2020
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourceScientific Reports
dc.subject.meshChromatography, Liquid / methods
dc.subject.meshCohort Studies
dc.subject.meshDisease Progression
dc.subject.meshEye Proteins / metabolism
dc.subject.meshHumans
dc.subject.meshImmunoglobulins / metabolism
dc.subject.meshKeratoconus / metabolism
dc.subject.meshTears / metabolism
dc.titleCharacterization of Tear Immunoglobulins in a Small-Cohort of Keratoconus Patients
dc.typeArticle
dc.type.materialtext

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